Characterization of the Bacillus subtilis spore morphogenetic coat protein CotO

D. C. McPherson, H. Kim, M. Hahn, R. Wang, P. Grabowski, P. Eichenberger, A. Driks

Research output: Contribution to journalArticle

Abstract

Bacillus spores are protected by a structurally and biochemically complex protein shell composed of over 50 polypeptide species, called the coat. Coat assembly in Bacillus subtilis serves as a relatively tractable model for the study of the formation of more complex macromolecular structures and organelles. It is also a critical model for the discovery of strategies to decontaminate B. anthracis spores. In B. subtilis, a subset of coat proteins is known to have important roles in assembly. Here we show that the recently identified B. subtilis coat protein CotO (YjbX) has an especially important morphogenetic role. We used electron and atomic force microscopy to show that CotO controls assembly of the coat layers and coat surface topography as well as biochemical and cell-biological analyses to identify coat proteins whose assembly is CotO dependent. cotO spores are defective in germination and partially sensitive to lysozyme. As a whole, these phenotypes resemble those resulting from a mutation in the coat protein gene cotH. Nonetheless, the roles of CotH and CotO and the proteins whose assembly they direct are not identical. Based on fluorescence and electron microscopy, we suggest that CotO resides in the outer coat (although not on the coat surface). We propose that CotO and CotH participate in a late phase of coat assembly. We further speculate that an important role of these proteins is ensuring that polymerization of the outer coat layers occurs in such a manner that contiguous shells, and not unproductive aggregates, are formed.

Original languageEnglish (US)
Pages (from-to)8278-8290
Number of pages13
JournalJournal of Bacteriology
Volume187
Issue number24
DOIs
StatePublished - Dec 2005

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Capsid Proteins
Bacillus subtilis
Spores
Macromolecular Substances
Proteins
Atomic Force Microscopy
Muramidase
Germination
Fluorescence Microscopy
Polymerization
Organelles
Bacillus
Electron Microscopy
Electrons
Phenotype
Peptides
Mutation
Genes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Characterization of the Bacillus subtilis spore morphogenetic coat protein CotO. / McPherson, D. C.; Kim, H.; Hahn, M.; Wang, R.; Grabowski, P.; Eichenberger, P.; Driks, A.

In: Journal of Bacteriology, Vol. 187, No. 24, 12.2005, p. 8278-8290.

Research output: Contribution to journalArticle

McPherson, D. C. ; Kim, H. ; Hahn, M. ; Wang, R. ; Grabowski, P. ; Eichenberger, P. ; Driks, A. / Characterization of the Bacillus subtilis spore morphogenetic coat protein CotO. In: Journal of Bacteriology. 2005 ; Vol. 187, No. 24. pp. 8278-8290.
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