Characterization of specific binding sites labeled by [3H]LSD in coronal sections of paraformaldehyde-fixed rat brain

Sheryl G. Beck, Efrain Azmitia

Research output: Contribution to journalArticle

Abstract

Specific, high-affinity binding of [3H]lysergic acid diethylamide (LSD) in coronal sections of paraformaldehyde-fixed rat brain is described. Intracardiac perfusion of paraformaldehyde selectively altered 40% of the total binding sites normally labeled by [3H]LSD in unfixed sections. Competition by unlabeled LSD and serotonin (5-HT) was not altered by the fixation procedure. Competition by spiperone, however, revealed that the fixation procedure preferentially altered sites for which spiperone has high affinity. This technique should facilitate the combination of neuroanatomical techniques such as radioautography and immunocytochemistry.

Original languageEnglish (US)
Pages (from-to)369-373
Number of pages5
JournalBrain Research
Volume345
Issue number2
DOIs
StatePublished - Oct 21 1985

Fingerprint

Lysergic Acid Diethylamide
Spiperone
Binding Sites
Serotonin
Brain
Autoradiography
Perfusion
Immunohistochemistry
paraform

Keywords

  • 5-hydroxytryptamine (5-HT)
  • binding site
  • paraformaldehyde fixation
  • spiperone
  • [H]lysergic acid diethylamide ([H]LSD)

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Characterization of specific binding sites labeled by [3H]LSD in coronal sections of paraformaldehyde-fixed rat brain. / Beck, Sheryl G.; Azmitia, Efrain.

In: Brain Research, Vol. 345, No. 2, 21.10.1985, p. 369-373.

Research output: Contribution to journalArticle

@article{119c1a28a3ac4aec85ce5bc74051f157,
title = "Characterization of specific binding sites labeled by [3H]LSD in coronal sections of paraformaldehyde-fixed rat brain",
abstract = "Specific, high-affinity binding of [3H]lysergic acid diethylamide (LSD) in coronal sections of paraformaldehyde-fixed rat brain is described. Intracardiac perfusion of paraformaldehyde selectively altered 40{\%} of the total binding sites normally labeled by [3H]LSD in unfixed sections. Competition by unlabeled LSD and serotonin (5-HT) was not altered by the fixation procedure. Competition by spiperone, however, revealed that the fixation procedure preferentially altered sites for which spiperone has high affinity. This technique should facilitate the combination of neuroanatomical techniques such as radioautography and immunocytochemistry.",
keywords = "5-hydroxytryptamine (5-HT), binding site, paraformaldehyde fixation, spiperone, [H]lysergic acid diethylamide ([H]LSD)",
author = "Beck, {Sheryl G.} and Efrain Azmitia",
year = "1985",
month = "10",
day = "21",
doi = "10.1016/0006-8993(85)91019-4",
language = "English (US)",
volume = "345",
pages = "369--373",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Characterization of specific binding sites labeled by [3H]LSD in coronal sections of paraformaldehyde-fixed rat brain

AU - Beck, Sheryl G.

AU - Azmitia, Efrain

PY - 1985/10/21

Y1 - 1985/10/21

N2 - Specific, high-affinity binding of [3H]lysergic acid diethylamide (LSD) in coronal sections of paraformaldehyde-fixed rat brain is described. Intracardiac perfusion of paraformaldehyde selectively altered 40% of the total binding sites normally labeled by [3H]LSD in unfixed sections. Competition by unlabeled LSD and serotonin (5-HT) was not altered by the fixation procedure. Competition by spiperone, however, revealed that the fixation procedure preferentially altered sites for which spiperone has high affinity. This technique should facilitate the combination of neuroanatomical techniques such as radioautography and immunocytochemistry.

AB - Specific, high-affinity binding of [3H]lysergic acid diethylamide (LSD) in coronal sections of paraformaldehyde-fixed rat brain is described. Intracardiac perfusion of paraformaldehyde selectively altered 40% of the total binding sites normally labeled by [3H]LSD in unfixed sections. Competition by unlabeled LSD and serotonin (5-HT) was not altered by the fixation procedure. Competition by spiperone, however, revealed that the fixation procedure preferentially altered sites for which spiperone has high affinity. This technique should facilitate the combination of neuroanatomical techniques such as radioautography and immunocytochemistry.

KW - 5-hydroxytryptamine (5-HT)

KW - binding site

KW - paraformaldehyde fixation

KW - spiperone

KW - [H]lysergic acid diethylamide ([H]LSD)

UR - http://www.scopus.com/inward/record.url?scp=0022414161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022414161&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(85)91019-4

DO - 10.1016/0006-8993(85)91019-4

M3 - Article

VL - 345

SP - 369

EP - 373

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -