Characterization of Pax3 and Sox10 transgenic Xenopus laevis embryos as tools to study neural crest development

Mansour Alkobtawi, Heather Ray, Elias H. Barriga, Mauricio Moreno, Ryan Kerney, Anne Helene Monsoro-Burq, Jean-Pierre Saint-Jeannet, Roberto Mayor

Research output: Contribution to journalArticle

Abstract

The neural crest is a multipotent population of cells that originates a variety of cell types. Many animal models are used to study neural crest induction, migration and differentiation, with amphibians and birds being the most widely used systems. A major technological advance to study neural crest development in mouse, chick and zebrafish has been the generation of transgenic animals in which neural crest specific enhancers/promoters drive the expression of either fluorescent proteins for use as lineage tracers, or modified genes for use in functional studies. Unfortunately, no such transgenic animals currently exist for the amphibians Xenopus laevis and tropicalis, key model systems for studying neural crest development. Here we describe the generation and characterization of two transgenic Xenopus laevis lines, Pax3-GFP and Sox10-GFP, in which GFP is expressed in the pre-migratory and migratory neural crest, respectively. We show that Pax3-GFP could be a powerful tool to study neural crest induction, whereas Sox10-GFP could be used in the study of neural crest migration in living embryos.

Original languageEnglish (US)
JournalDevelopmental Biology
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Neural Crest
Xenopus laevis
Embryonic Structures
Genetically Modified Animals
Amphibians
Zebrafish
Birds
Animal Models

Keywords

  • induction
  • migration
  • Neural crest
  • Pax3
  • Sox10
  • transgenic

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Alkobtawi, M., Ray, H., Barriga, E. H., Moreno, M., Kerney, R., Monsoro-Burq, A. H., ... Mayor, R. (Accepted/In press). Characterization of Pax3 and Sox10 transgenic Xenopus laevis embryos as tools to study neural crest development. Developmental Biology. https://doi.org/10.1016/j.ydbio.2018.02.020

Characterization of Pax3 and Sox10 transgenic Xenopus laevis embryos as tools to study neural crest development. / Alkobtawi, Mansour; Ray, Heather; Barriga, Elias H.; Moreno, Mauricio; Kerney, Ryan; Monsoro-Burq, Anne Helene; Saint-Jeannet, Jean-Pierre; Mayor, Roberto.

In: Developmental Biology, 01.01.2018.

Research output: Contribution to journalArticle

Alkobtawi, Mansour ; Ray, Heather ; Barriga, Elias H. ; Moreno, Mauricio ; Kerney, Ryan ; Monsoro-Burq, Anne Helene ; Saint-Jeannet, Jean-Pierre ; Mayor, Roberto. / Characterization of Pax3 and Sox10 transgenic Xenopus laevis embryos as tools to study neural crest development. In: Developmental Biology. 2018.
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