Characterization of membrane materials and membrane coatings for bioreactor units of bioartificial kidneys

Ming Ni, Jeremy Teo, Mohammed Shahrudin bin Ibrahim, Kangyi Zhang, Farah Tasnim, Pei Yong Chow, Daniele Zink, Jackie Y. Ying

Research output: Contribution to journalArticle

Abstract

The bioreactor unit of bioartificial kidneys contains porous membranes seeded with renal cells. For clinical applications, it is mandatory that human primary renal proximal tubule cells (HPTCs) form differentiated epithelia on the membranes. Here, we show that HPTCs do not grow and survive on a variety of polymeric membrane materials. This applies also to membranes consisting of polysulfone/polyvinylpyrrolidone (PSF/PVP), which have been used in the bioreactor unit of bioartificial kidneys after coating with an extracellular matrix (ECM). Our data reveal that coating with just an ECM does not sufficiently improve HPTC performance on non-HPTC-compatible membrane materials. On the other hand, we have characterized the effects of a variety of surface treatments and coatings, and found that double coating with 3,4-dihydroxy- l-phenylalanine and an ECM markedly improves HPTC performance and results in the formation of differentiated epithelia on PSF/PVP membranes. We have also synthesized alternative membrane materials, and characterized membranes consisting of polysulfone and Fullcure™. We found that these membranes sustain proper HPTC performance without the need for surface treatments or coatings. Together, our data reveal that the materials that have been previously applied in bioartificial kidneys are not suitable for applications with HPTCs. This study elucidates the types of membrane materials and coatings that are favorable for the bioreactor unit of bioartificial kidneys.

Original languageEnglish (US)
Pages (from-to)1465-1476
Number of pages12
JournalBiomaterials
Volume32
Issue number6
DOIs
StatePublished - Feb 1 2011

Fingerprint

Bioreactors
Proximal Kidney Tubule
Membranes
Kidney
Coatings
Polysulfones
Extracellular Matrix
Povidone
Surface treatment
Epithelium
Polymeric membranes
Phenylalanine
Cell Membrane

Keywords

  • Biocompatibility
  • Bioreactor
  • Epithelial cell
  • Membrane
  • Polysulphone
  • Surface treatment

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Characterization of membrane materials and membrane coatings for bioreactor units of bioartificial kidneys. / Ni, Ming; Teo, Jeremy; Ibrahim, Mohammed Shahrudin bin; Zhang, Kangyi; Tasnim, Farah; Chow, Pei Yong; Zink, Daniele; Ying, Jackie Y.

In: Biomaterials, Vol. 32, No. 6, 01.02.2011, p. 1465-1476.

Research output: Contribution to journalArticle

Ni, Ming ; Teo, Jeremy ; Ibrahim, Mohammed Shahrudin bin ; Zhang, Kangyi ; Tasnim, Farah ; Chow, Pei Yong ; Zink, Daniele ; Ying, Jackie Y. / Characterization of membrane materials and membrane coatings for bioreactor units of bioartificial kidneys. In: Biomaterials. 2011 ; Vol. 32, No. 6. pp. 1465-1476.
@article{330949281ad04e0098e89f9f9c2bc252,
title = "Characterization of membrane materials and membrane coatings for bioreactor units of bioartificial kidneys",
abstract = "The bioreactor unit of bioartificial kidneys contains porous membranes seeded with renal cells. For clinical applications, it is mandatory that human primary renal proximal tubule cells (HPTCs) form differentiated epithelia on the membranes. Here, we show that HPTCs do not grow and survive on a variety of polymeric membrane materials. This applies also to membranes consisting of polysulfone/polyvinylpyrrolidone (PSF/PVP), which have been used in the bioreactor unit of bioartificial kidneys after coating with an extracellular matrix (ECM). Our data reveal that coating with just an ECM does not sufficiently improve HPTC performance on non-HPTC-compatible membrane materials. On the other hand, we have characterized the effects of a variety of surface treatments and coatings, and found that double coating with 3,4-dihydroxy- l-phenylalanine and an ECM markedly improves HPTC performance and results in the formation of differentiated epithelia on PSF/PVP membranes. We have also synthesized alternative membrane materials, and characterized membranes consisting of polysulfone and Fullcure™. We found that these membranes sustain proper HPTC performance without the need for surface treatments or coatings. Together, our data reveal that the materials that have been previously applied in bioartificial kidneys are not suitable for applications with HPTCs. This study elucidates the types of membrane materials and coatings that are favorable for the bioreactor unit of bioartificial kidneys.",
keywords = "Biocompatibility, Bioreactor, Epithelial cell, Membrane, Polysulphone, Surface treatment",
author = "Ming Ni and Jeremy Teo and Ibrahim, {Mohammed Shahrudin bin} and Kangyi Zhang and Farah Tasnim and Chow, {Pei Yong} and Daniele Zink and Ying, {Jackie Y.}",
year = "2011",
month = "2",
day = "1",
doi = "10.1016/j.biomaterials.2010.10.061",
language = "English (US)",
volume = "32",
pages = "1465--1476",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "6",

}

TY - JOUR

T1 - Characterization of membrane materials and membrane coatings for bioreactor units of bioartificial kidneys

AU - Ni, Ming

AU - Teo, Jeremy

AU - Ibrahim, Mohammed Shahrudin bin

AU - Zhang, Kangyi

AU - Tasnim, Farah

AU - Chow, Pei Yong

AU - Zink, Daniele

AU - Ying, Jackie Y.

PY - 2011/2/1

Y1 - 2011/2/1

N2 - The bioreactor unit of bioartificial kidneys contains porous membranes seeded with renal cells. For clinical applications, it is mandatory that human primary renal proximal tubule cells (HPTCs) form differentiated epithelia on the membranes. Here, we show that HPTCs do not grow and survive on a variety of polymeric membrane materials. This applies also to membranes consisting of polysulfone/polyvinylpyrrolidone (PSF/PVP), which have been used in the bioreactor unit of bioartificial kidneys after coating with an extracellular matrix (ECM). Our data reveal that coating with just an ECM does not sufficiently improve HPTC performance on non-HPTC-compatible membrane materials. On the other hand, we have characterized the effects of a variety of surface treatments and coatings, and found that double coating with 3,4-dihydroxy- l-phenylalanine and an ECM markedly improves HPTC performance and results in the formation of differentiated epithelia on PSF/PVP membranes. We have also synthesized alternative membrane materials, and characterized membranes consisting of polysulfone and Fullcure™. We found that these membranes sustain proper HPTC performance without the need for surface treatments or coatings. Together, our data reveal that the materials that have been previously applied in bioartificial kidneys are not suitable for applications with HPTCs. This study elucidates the types of membrane materials and coatings that are favorable for the bioreactor unit of bioartificial kidneys.

AB - The bioreactor unit of bioartificial kidneys contains porous membranes seeded with renal cells. For clinical applications, it is mandatory that human primary renal proximal tubule cells (HPTCs) form differentiated epithelia on the membranes. Here, we show that HPTCs do not grow and survive on a variety of polymeric membrane materials. This applies also to membranes consisting of polysulfone/polyvinylpyrrolidone (PSF/PVP), which have been used in the bioreactor unit of bioartificial kidneys after coating with an extracellular matrix (ECM). Our data reveal that coating with just an ECM does not sufficiently improve HPTC performance on non-HPTC-compatible membrane materials. On the other hand, we have characterized the effects of a variety of surface treatments and coatings, and found that double coating with 3,4-dihydroxy- l-phenylalanine and an ECM markedly improves HPTC performance and results in the formation of differentiated epithelia on PSF/PVP membranes. We have also synthesized alternative membrane materials, and characterized membranes consisting of polysulfone and Fullcure™. We found that these membranes sustain proper HPTC performance without the need for surface treatments or coatings. Together, our data reveal that the materials that have been previously applied in bioartificial kidneys are not suitable for applications with HPTCs. This study elucidates the types of membrane materials and coatings that are favorable for the bioreactor unit of bioartificial kidneys.

KW - Biocompatibility

KW - Bioreactor

KW - Epithelial cell

KW - Membrane

KW - Polysulphone

KW - Surface treatment

UR - http://www.scopus.com/inward/record.url?scp=78650283186&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650283186&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2010.10.061

DO - 10.1016/j.biomaterials.2010.10.061

M3 - Article

C2 - 21145586

AN - SCOPUS:78650283186

VL - 32

SP - 1465

EP - 1476

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 6

ER -