Characterization of human fungiform papillae cells in culture.

Mehmet Hakan Ozdener, Joseph G. Brand, Andrew I. Spielman, Fritz W. Lischka, John H. Teeter, Paul A S Breslin, Nancy E. Rawson

Research output: Contribution to journalArticle

Abstract

The ability to maintain human fungiform papillae cells in culture for multiple cell cycles would be of considerable utility for characterizing the molecular, regenerative, and functional properties of these unique sensory cells. Here we describe a method for enzymatically isolating human cells from fungiform papillae obtained by biopsy and maintaining them in culture for more than 7 passages (7 months) without loss of viability and while retaining many of the functional properties of acutely isolated taste cells. Cells in these cultures exhibited increases in intracellular calcium when stimulated with perceptually appropriate concentrations of several taste stimuli, indicating that at least some of the native signaling pathways were present. This system can provide a useful model for molecular studies of the proliferation, differentiation, and physiological function of human fungiform papillae cells.

Original languageEnglish (US)
Pages (from-to)601-612
Number of pages12
JournalChemical Senses
Volume36
Issue number7
StatePublished - Sep 2011

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Cell Culture Techniques
Molecular Models
Aptitude
Cell Cycle
Calcium
Biopsy

ASJC Scopus subject areas

  • Sensory Systems
  • Behavioral Neuroscience
  • Physiology
  • Physiology (medical)

Cite this

Ozdener, M. H., Brand, J. G., Spielman, A. I., Lischka, F. W., Teeter, J. H., Breslin, P. A. S., & Rawson, N. E. (2011). Characterization of human fungiform papillae cells in culture. Chemical Senses, 36(7), 601-612.

Characterization of human fungiform papillae cells in culture. / Ozdener, Mehmet Hakan; Brand, Joseph G.; Spielman, Andrew I.; Lischka, Fritz W.; Teeter, John H.; Breslin, Paul A S; Rawson, Nancy E.

In: Chemical Senses, Vol. 36, No. 7, 09.2011, p. 601-612.

Research output: Contribution to journalArticle

Ozdener, MH, Brand, JG, Spielman, AI, Lischka, FW, Teeter, JH, Breslin, PAS & Rawson, NE 2011, 'Characterization of human fungiform papillae cells in culture.', Chemical Senses, vol. 36, no. 7, pp. 601-612.
Ozdener MH, Brand JG, Spielman AI, Lischka FW, Teeter JH, Breslin PAS et al. Characterization of human fungiform papillae cells in culture. Chemical Senses. 2011 Sep;36(7):601-612.
Ozdener, Mehmet Hakan ; Brand, Joseph G. ; Spielman, Andrew I. ; Lischka, Fritz W. ; Teeter, John H. ; Breslin, Paul A S ; Rawson, Nancy E. / Characterization of human fungiform papillae cells in culture. In: Chemical Senses. 2011 ; Vol. 36, No. 7. pp. 601-612.
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