Characterization of genipin-modified dentin collagen

Hiroko Nagaoka, Hideaki Nagaoka, Ricardo Walter, Lee W. Boushell, Patricia A. Miguez, Andrew Burton, André V. Ritter, Mitsuo Yamauchi

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Abstract

Application of biomodification techniques to dentin can improve its biochemical and biomechanical properties. Several collagen cross-linking agents have been reported to strengthen the mechanical properties of dentin. However, the characteristics of collagen that has undergone agent-induced biomodification are not well understood. The objective of this study was to analyze the effects of a natural cross-linking agent, genipin (GE), on dentin discoloration, collagen stability, and changes in amino acid composition and lysyl oxidase mediated natural collagen cross-links. Dentin collagen obtained from extracted bovine teeth was treated with three different concentrations of GE (0.01%, 0.1%, and 0.5%) for several treatment times (0-24 h). Changes in biochemical properties of NaB3H4-reduced collagen were characterized by amino acid and cross-link analyses. The treatment of dentin collagen with GE resulted in a concentration- and time-dependent pigmentation and stability against bacterial collagenase. The lysyl oxidase-mediated trivalent mature cross-link, pyridinoline, showed no difference among all groups while the major divalent immature cross-link, dehydro-dihydroxylysinonorleucine/its ketoamine in collagen treated with 0.5% GE for 24 h, significantly decreased compared to control (P < 0.05). The newly formed GE-induced cross-links most likely involve lysine and hydroxylysine residues of collagen in a concentration- dependent manner. Some of these cross-links appear to be reducible and stabilized with NaB3H4.

Original languageEnglish (US)
Article number702821
JournalBioMed Research International
Volume2014
DOIs
StatePublished - 2014

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Nagaoka, H., Nagaoka, H., Walter, R., Boushell, L. W., Miguez, P. A., Burton, A., Ritter, A. V., & Yamauchi, M. (2014). Characterization of genipin-modified dentin collagen. BioMed Research International, 2014, [702821]. https://doi.org/10.1155/2014/702821