Characterization of a lymphokine produced by human T cells which inhibits collagen synthesis

Joel Rosenbloom, William McArthur, Daniel Malamud, Sergio Jimenez

Research output: Contribution to journalArticle

Abstract

Human lymphocytes, isolated from peripheral blood, were cultured for 48 hr in a defined medium containing 10 mg/ml bovine serum albumin and phytohemagglutinin. A lymphokine which inhibits collagen synthesis by cultured human dermal fibroblasts was purified from the lymphocyte incubation medium by successive steps of ammonium sulfate precipitation, gel filtration chromatography, and isoelectric focusing. Good recovery of this collagen synthesis inhibitory factor (CSIF) was obtained and a factor with an approximate molecular weight of 55,000 and a pI of 6.2 was isolated. The purification of the factor should permit further studies on its mechanism of action.

Original languageEnglish (US)
Pages (from-to)192-198
Number of pages7
JournalCellular Immunology
Volume81
Issue number1
DOIs
StatePublished - Oct 1 1983

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ASJC Scopus subject areas

  • Immunology

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