Characterization of a Bacillus anthracis spore coat-surface protein that influences coat-surface morphology

Michael Mallozzi, Joel Bozue, Rebecca Giorno, Krishna Sulayman Moody, Alex Slack, Christopher Cote, Dengli Qiu, Rong Wang, Peter McKenney, Erh Min Lai, Janine R. Maddock, Arthur Friedlander, Susan Welkos, Patrick Eichenberger, Adam Driks

Research output: Contribution to journalArticle

Abstract

Bacterial spores are encased in a multilayered proteinaceous shell, called the coat. In many Bacillus spp., the coat protects against environmental assault and facilitates germination. In Bacillus anthracis, the spore is the etiological agent of anthrax, and the functions of the coat likely contribute to virulence. Here, we characterize a B. anthracis spore protein, called Cotβ, which is encoded only in the genomes of the Bacillus cereus group. We found that Cotβ is synthesized specifically during sporulation and is assembled onto the spore coat surface. Our analysis of a cotβ null mutant in the Sterne strain reveals that Cotβ has a role in determining coat-surface morphology but does not detectably affect germination. In the fully virulent Ames strain, a cotβ null mutation has no effect on virulence in a murine model of B. anthracis infection.

Original languageEnglish (US)
Pages (from-to)110-117
Number of pages8
JournalFEMS Microbiology Letters
Volume289
Issue number1
DOIs
StatePublished - Dec 2008

Fingerprint

Bacillus anthracis
Capsid Proteins
Spores
Membrane Proteins
Germination
Virulence
Bacterial Spores
Anthrax
Bacillus cereus
Bacillus
Genome
Mutation
Infection
Proteins

Keywords

  • Anthrax
  • Bacillus anthracis
  • Coat
  • Germination
  • Spore assembly

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

Characterization of a Bacillus anthracis spore coat-surface protein that influences coat-surface morphology. / Mallozzi, Michael; Bozue, Joel; Giorno, Rebecca; Moody, Krishna Sulayman; Slack, Alex; Cote, Christopher; Qiu, Dengli; Wang, Rong; McKenney, Peter; Lai, Erh Min; Maddock, Janine R.; Friedlander, Arthur; Welkos, Susan; Eichenberger, Patrick; Driks, Adam.

In: FEMS Microbiology Letters, Vol. 289, No. 1, 12.2008, p. 110-117.

Research output: Contribution to journalArticle

Mallozzi, M, Bozue, J, Giorno, R, Moody, KS, Slack, A, Cote, C, Qiu, D, Wang, R, McKenney, P, Lai, EM, Maddock, JR, Friedlander, A, Welkos, S, Eichenberger, P & Driks, A 2008, 'Characterization of a Bacillus anthracis spore coat-surface protein that influences coat-surface morphology', FEMS Microbiology Letters, vol. 289, no. 1, pp. 110-117. https://doi.org/10.1111/j.1574-6968.2008.01380.x
Mallozzi, Michael ; Bozue, Joel ; Giorno, Rebecca ; Moody, Krishna Sulayman ; Slack, Alex ; Cote, Christopher ; Qiu, Dengli ; Wang, Rong ; McKenney, Peter ; Lai, Erh Min ; Maddock, Janine R. ; Friedlander, Arthur ; Welkos, Susan ; Eichenberger, Patrick ; Driks, Adam. / Characterization of a Bacillus anthracis spore coat-surface protein that influences coat-surface morphology. In: FEMS Microbiology Letters. 2008 ; Vol. 289, No. 1. pp. 110-117.
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