Characterization and localization of the even-skipped protein of Drosophila.

M. Frasch, T. Hoey, Christine Rushlow, H. Doyle, M. Levine

Research output: Contribution to journalArticle

Abstract

On the basis of homeo box cross-homology we have isolated the pair-rule gene even-skipped (eve) of Drosophila. The eve transcription unit appears to be less than 1.5 kb in length, and encodes a single mRNA of approximately 1.4 kb. The nucleotide sequence of genomic and cDNA clones indicates that the eve protein is composed of 376 amino acid residues, and that its homeo domain shares only approximately 50% amino acid identity with the homeo domains of previously characterized genes. Using antibodies raised against a beta-galactosidase fusion protein we show that the eve protein is distributed in a series of seven transverse stripes at the cellular blastoderm stage, and is localized primarily within the nuclear regions of those embryonic cells that express the gene. After gastrulation, seven weakly stained stripes of eve expression appear, resulting in a transient pattern that consists of a total of 14 evenly spaced stripes. Both the original and new stripes gradually disappear during germ band elongation. A second expression pattern emerges during neurogenesis, whereby eve protein is detected in discrete subsets of neurons in each of the ventral ganglia.

Original languageEnglish (US)
Pages (from-to)749-759
Number of pages11
JournalEMBO Journal
Volume6
Issue number3
StatePublished - Mar 1987

Fingerprint

Genes
Proteins
Blastoderm
Amino Acids
Gastrulation
Homeobox Genes
Neurogenesis
Transcription
beta-Galactosidase
Ganglia
Drosophila
Neurons
Elongation
Fusion reactions
Nucleotides
Complementary DNA
Clone Cells
Messenger RNA
Drosophila eve protein
Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Frasch, M., Hoey, T., Rushlow, C., Doyle, H., & Levine, M. (1987). Characterization and localization of the even-skipped protein of Drosophila. EMBO Journal, 6(3), 749-759.

Characterization and localization of the even-skipped protein of Drosophila. / Frasch, M.; Hoey, T.; Rushlow, Christine; Doyle, H.; Levine, M.

In: EMBO Journal, Vol. 6, No. 3, 03.1987, p. 749-759.

Research output: Contribution to journalArticle

Frasch, M, Hoey, T, Rushlow, C, Doyle, H & Levine, M 1987, 'Characterization and localization of the even-skipped protein of Drosophila.', EMBO Journal, vol. 6, no. 3, pp. 749-759.
Frasch, M. ; Hoey, T. ; Rushlow, Christine ; Doyle, H. ; Levine, M. / Characterization and localization of the even-skipped protein of Drosophila. In: EMBO Journal. 1987 ; Vol. 6, No. 3. pp. 749-759.
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