Channel modulation and the mechanism of light adaptation in mouse rods

Jeannie Chen, Michael L. Woodruff, Tian Wang, Francis A. Concepcion, Daniel Tranchina, Gordon L. Fain

Research output: Contribution to journalArticle

Abstract

Vertebrate photoreceptors are thought to adapt to light by a change in Ca2+, which is postulated to mediate modulation of (1) excited rhodopsin (Rh*) by Ca2+-dependent binding of recoverin, (2) guanylyl cyclase activity via Ca2+-dependent GCAP proteins, and (3) cyclic nucleotide-gated channels by binding of Ca2+-calmodulin. Previous experiments genetically deleted recoverin and the GCAPs and showed that significant regulation of sensitivity survives removal of (1) and (2). We genetically deleted the channel Ca2+-calmodulin binding site in the mouse Mus musculus and found that removal of (3) alters response waveform, but removal of (3) or of (2) and (3) together still leaves much of adaptation intact. These experiments demonstrate that an important additional mechanism is required, which other experiments indicate may be regulation of phosphodiesterase 6 (PDE6). We therefore constructed a kinetic model in which light produces a Ca2+-mediated decrease in PDE6 decay rate, with the novel feature that both spontaneously activated and light-activated PDE6 are modulated. This model, together with Ca2+-dependent acceleration of guanylyl cyclase, can successfully account for changes in sensitivity and response waveform in background light. Copyright

Original languageEnglish (US)
Pages (from-to)16232-16240
Number of pages9
JournalJournal of Neuroscience
Volume30
Issue number48
DOIs
StatePublished - Dec 1 2010

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Ocular Adaptation
Type 6 Cyclic Nucleotide Phosphodiesterases
Recoverin
Light
Guanylate Cyclase
Calmodulin
Guanylate Cyclase-Activating Proteins
Cyclic Nucleotide-Gated Cation Channels
Vertebrate Photoreceptor Cells
Rhodopsin
Binding Sites

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Chen, J., Woodruff, M. L., Wang, T., Concepcion, F. A., Tranchina, D., & Fain, G. L. (2010). Channel modulation and the mechanism of light adaptation in mouse rods. Journal of Neuroscience, 30(48), 16232-16240. https://doi.org/10.1523/JNEUROSCI.2868-10.2010

Channel modulation and the mechanism of light adaptation in mouse rods. / Chen, Jeannie; Woodruff, Michael L.; Wang, Tian; Concepcion, Francis A.; Tranchina, Daniel; Fain, Gordon L.

In: Journal of Neuroscience, Vol. 30, No. 48, 01.12.2010, p. 16232-16240.

Research output: Contribution to journalArticle

Chen, J, Woodruff, ML, Wang, T, Concepcion, FA, Tranchina, D & Fain, GL 2010, 'Channel modulation and the mechanism of light adaptation in mouse rods', Journal of Neuroscience, vol. 30, no. 48, pp. 16232-16240. https://doi.org/10.1523/JNEUROSCI.2868-10.2010
Chen, Jeannie ; Woodruff, Michael L. ; Wang, Tian ; Concepcion, Francis A. ; Tranchina, Daniel ; Fain, Gordon L. / Channel modulation and the mechanism of light adaptation in mouse rods. In: Journal of Neuroscience. 2010 ; Vol. 30, No. 48. pp. 16232-16240.
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