Changes in matrix protein gene expression associated with mineralization in the differentiating chick limb-bud micromass culture system

Cristina Teixeira, Jenny Xiang, Rani Roy, Valery Kudrashov, Itzhak Binderman, Philipp Mayer-Kuckuk, Adele L. Boskey

Research output: Contribution to journalArticle

Abstract

Chick limb-bud mesenchymal stem cells plated in high density culture in the presence of 4mM inorganic phosphate and vitamin C differentiate and form a mineralizable matrix, resembling that of the chick growth plate. To further elucidate the mechanism that allows these cultures to form physiologic hydroxyapatite deposits, and how the process can be manipulated to gain insight into mineralization mechanisms, we compared gene expression in mineralizing (with 4mM inorganic phosphate) and non-mineralizing cultures (containing only 1mM inorganic phosphate) at the start of mineralization (day 11) and after mineralization reached a plateau (day 17) using a chick specific microarray. Based on replicate microarray experiments and K-cluster analysis, several genes associated with the mineralization process were identified, and their expression patterns confirmed throughout the culture period by quantitative RT-PCR. The functions of bone morphogenetic protein 1, BMP1, dentin matrix protein 1, DMP1, the sodium phosphate co-transporter, NaPi IIb, matrix metalloprotease 13. MMP-13, and alkaline phosphatase, along with matrix protein genes (type X collagen, bone sialoprotein, and osteopontin) usually associated with initiation of mineralization are discussed.

Original languageEnglish (US)
Pages (from-to)607-613
Number of pages7
JournalJournal of Cellular Biochemistry
Volume112
Issue number2
DOIs
StatePublished - Feb 2011

Fingerprint

Limb Buds
Cell culture
Gene expression
Phosphates
Gene Expression
Bone Morphogenetic Protein 1
Sodium-Phosphate Cotransporter Proteins
Microarrays
Collagen Type X
Integrin-Binding Sialoprotein
Symporters
Proteins
Osteopontin
Growth Plate
Metalloproteases
Dentin
Durapatite
Matrix Metalloproteinases
Mesenchymal Stromal Cells
Ascorbic Acid

Keywords

  • chick limb-bud
  • gene expression
  • microarray
  • micromass culture
  • mineralization

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Changes in matrix protein gene expression associated with mineralization in the differentiating chick limb-bud micromass culture system. / Teixeira, Cristina; Xiang, Jenny; Roy, Rani; Kudrashov, Valery; Binderman, Itzhak; Mayer-Kuckuk, Philipp; Boskey, Adele L.

In: Journal of Cellular Biochemistry, Vol. 112, No. 2, 02.2011, p. 607-613.

Research output: Contribution to journalArticle

Teixeira, Cristina ; Xiang, Jenny ; Roy, Rani ; Kudrashov, Valery ; Binderman, Itzhak ; Mayer-Kuckuk, Philipp ; Boskey, Adele L. / Changes in matrix protein gene expression associated with mineralization in the differentiating chick limb-bud micromass culture system. In: Journal of Cellular Biochemistry. 2011 ; Vol. 112, No. 2. pp. 607-613.
@article{c78b11c8ea844ceebfc3f5c9492b88d8,
title = "Changes in matrix protein gene expression associated with mineralization in the differentiating chick limb-bud micromass culture system",
abstract = "Chick limb-bud mesenchymal stem cells plated in high density culture in the presence of 4mM inorganic phosphate and vitamin C differentiate and form a mineralizable matrix, resembling that of the chick growth plate. To further elucidate the mechanism that allows these cultures to form physiologic hydroxyapatite deposits, and how the process can be manipulated to gain insight into mineralization mechanisms, we compared gene expression in mineralizing (with 4mM inorganic phosphate) and non-mineralizing cultures (containing only 1mM inorganic phosphate) at the start of mineralization (day 11) and after mineralization reached a plateau (day 17) using a chick specific microarray. Based on replicate microarray experiments and K-cluster analysis, several genes associated with the mineralization process were identified, and their expression patterns confirmed throughout the culture period by quantitative RT-PCR. The functions of bone morphogenetic protein 1, BMP1, dentin matrix protein 1, DMP1, the sodium phosphate co-transporter, NaPi IIb, matrix metalloprotease 13. MMP-13, and alkaline phosphatase, along with matrix protein genes (type X collagen, bone sialoprotein, and osteopontin) usually associated with initiation of mineralization are discussed.",
keywords = "chick limb-bud, gene expression, microarray, micromass culture, mineralization",
author = "Cristina Teixeira and Jenny Xiang and Rani Roy and Valery Kudrashov and Itzhak Binderman and Philipp Mayer-Kuckuk and Boskey, {Adele L.}",
year = "2011",
month = "2",
doi = "10.1002/jcb.22951",
language = "English (US)",
volume = "112",
pages = "607--613",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Changes in matrix protein gene expression associated with mineralization in the differentiating chick limb-bud micromass culture system

AU - Teixeira, Cristina

AU - Xiang, Jenny

AU - Roy, Rani

AU - Kudrashov, Valery

AU - Binderman, Itzhak

AU - Mayer-Kuckuk, Philipp

AU - Boskey, Adele L.

PY - 2011/2

Y1 - 2011/2

N2 - Chick limb-bud mesenchymal stem cells plated in high density culture in the presence of 4mM inorganic phosphate and vitamin C differentiate and form a mineralizable matrix, resembling that of the chick growth plate. To further elucidate the mechanism that allows these cultures to form physiologic hydroxyapatite deposits, and how the process can be manipulated to gain insight into mineralization mechanisms, we compared gene expression in mineralizing (with 4mM inorganic phosphate) and non-mineralizing cultures (containing only 1mM inorganic phosphate) at the start of mineralization (day 11) and after mineralization reached a plateau (day 17) using a chick specific microarray. Based on replicate microarray experiments and K-cluster analysis, several genes associated with the mineralization process were identified, and their expression patterns confirmed throughout the culture period by quantitative RT-PCR. The functions of bone morphogenetic protein 1, BMP1, dentin matrix protein 1, DMP1, the sodium phosphate co-transporter, NaPi IIb, matrix metalloprotease 13. MMP-13, and alkaline phosphatase, along with matrix protein genes (type X collagen, bone sialoprotein, and osteopontin) usually associated with initiation of mineralization are discussed.

AB - Chick limb-bud mesenchymal stem cells plated in high density culture in the presence of 4mM inorganic phosphate and vitamin C differentiate and form a mineralizable matrix, resembling that of the chick growth plate. To further elucidate the mechanism that allows these cultures to form physiologic hydroxyapatite deposits, and how the process can be manipulated to gain insight into mineralization mechanisms, we compared gene expression in mineralizing (with 4mM inorganic phosphate) and non-mineralizing cultures (containing only 1mM inorganic phosphate) at the start of mineralization (day 11) and after mineralization reached a plateau (day 17) using a chick specific microarray. Based on replicate microarray experiments and K-cluster analysis, several genes associated with the mineralization process were identified, and their expression patterns confirmed throughout the culture period by quantitative RT-PCR. The functions of bone morphogenetic protein 1, BMP1, dentin matrix protein 1, DMP1, the sodium phosphate co-transporter, NaPi IIb, matrix metalloprotease 13. MMP-13, and alkaline phosphatase, along with matrix protein genes (type X collagen, bone sialoprotein, and osteopontin) usually associated with initiation of mineralization are discussed.

KW - chick limb-bud

KW - gene expression

KW - microarray

KW - micromass culture

KW - mineralization

UR - http://www.scopus.com/inward/record.url?scp=79251622447&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79251622447&partnerID=8YFLogxK

U2 - 10.1002/jcb.22951

DO - 10.1002/jcb.22951

M3 - Article

VL - 112

SP - 607

EP - 613

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 2

ER -