Changes in gene expression in space and time orchestrate environmentally mediated shaping of root architecture

Liam Walker, Clare Boddington, Dafyd Jenkins, Ying Wang, Jesper T. Grønlund, Jo Hulsmans, Sanjeev Kumar, Dhaval Patel, Jonathan D. Moore, Anthony Carter, Siva Samavedam, Giovanni Bonomo, David S. Hersh, Gloria Coruzzi, Nigel J. Burroughs, Miriam L. Gifford

Research output: Contribution to journalArticle

Abstract

Shaping of root architecture is a quintessential developmental response that involves the concerted action of many different cell types, is highly dynamic, and underpins root plasticity. To determine to what extent the environmental regulation of lateral root development is a product of cell-type preferential activities, we tracked transcriptomic responses to two different treatments that both change root development in Arabidopsis thaliana at an unprecedented level of temporal detail. We found that individual transcripts are expressed with a very high degree of temporal and spatial specificity, yet biological processes are commonly regulated, in a mechanism we term response nonredundancy. Using causative gene network inference to compare the genes regulated in different cell types and during responses to nitrogen and a biotic interaction, we found that common transcriptional modules often regulate the same gene families but control different individual members of these families, specific to response and cell type. This reinforces that the activity of a gene cannot be defined simply as molecular function; rather, it is a consequence of spatial location, expression timing, and environmental responsiveness.

Original languageEnglish (US)
Pages (from-to)2393-2412
Number of pages20
JournalPlant Cell
Volume29
Issue number10
DOIs
StatePublished - Oct 1 2017

Fingerprint

space and time
Gene Expression
gene expression
cells
Genes
Biological Phenomena
environmental law
genes
Gene Regulatory Networks
transcriptomics
Arabidopsis
Nitrogen
Arabidopsis thaliana
nitrogen

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Walker, L., Boddington, C., Jenkins, D., Wang, Y., Grønlund, J. T., Hulsmans, J., ... Gifford, M. L. (2017). Changes in gene expression in space and time orchestrate environmentally mediated shaping of root architecture. Plant Cell, 29(10), 2393-2412. https://doi.org/10.1105/tpc.16.00961

Changes in gene expression in space and time orchestrate environmentally mediated shaping of root architecture. / Walker, Liam; Boddington, Clare; Jenkins, Dafyd; Wang, Ying; Grønlund, Jesper T.; Hulsmans, Jo; Kumar, Sanjeev; Patel, Dhaval; Moore, Jonathan D.; Carter, Anthony; Samavedam, Siva; Bonomo, Giovanni; Hersh, David S.; Coruzzi, Gloria; Burroughs, Nigel J.; Gifford, Miriam L.

In: Plant Cell, Vol. 29, No. 10, 01.10.2017, p. 2393-2412.

Research output: Contribution to journalArticle

Walker, L, Boddington, C, Jenkins, D, Wang, Y, Grønlund, JT, Hulsmans, J, Kumar, S, Patel, D, Moore, JD, Carter, A, Samavedam, S, Bonomo, G, Hersh, DS, Coruzzi, G, Burroughs, NJ & Gifford, ML 2017, 'Changes in gene expression in space and time orchestrate environmentally mediated shaping of root architecture', Plant Cell, vol. 29, no. 10, pp. 2393-2412. https://doi.org/10.1105/tpc.16.00961
Walker L, Boddington C, Jenkins D, Wang Y, Grønlund JT, Hulsmans J et al. Changes in gene expression in space and time orchestrate environmentally mediated shaping of root architecture. Plant Cell. 2017 Oct 1;29(10):2393-2412. https://doi.org/10.1105/tpc.16.00961
Walker, Liam ; Boddington, Clare ; Jenkins, Dafyd ; Wang, Ying ; Grønlund, Jesper T. ; Hulsmans, Jo ; Kumar, Sanjeev ; Patel, Dhaval ; Moore, Jonathan D. ; Carter, Anthony ; Samavedam, Siva ; Bonomo, Giovanni ; Hersh, David S. ; Coruzzi, Gloria ; Burroughs, Nigel J. ; Gifford, Miriam L. / Changes in gene expression in space and time orchestrate environmentally mediated shaping of root architecture. In: Plant Cell. 2017 ; Vol. 29, No. 10. pp. 2393-2412.
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