Changes in abundance of oral microbiota associated with oral cancer

Brian Schmidt, Justin Kuczynski, Aditi Bhattacharya, Bing Huey, Patricia M. Corby, Erica L S Queiroz, Kira Nightingale, A. Ross Kerr, Mark D. DeLacure, Ratna Veeramachaneni, Adam B. Olshen, Donna Albertson

Research output: Contribution to journalArticle

Abstract

Individual bacteria and shifts in the composition of the microbiome have been associated with human diseases including cancer. To investigate changes in the microbiome associated with oral cancers, we profiled cancers and anatomically matched contralateral normal tissue from the same patient by sequencing 16S rDNA hypervariable region amplicons. In cancer samples from both a discovery and a subsequent confirmation cohort, abundance of Firmicutes (especially Streptococcus) and Actinobacteria (especially Rothia ) was significantly decreased relative to contralateral normal samples from the same patient. Significant decreases in abundance of these phyla were observed for pre-cancers, but not when comparing samples from contralateral sites (tongue and floor of mouth) from healthy individuals. Weighted UniFrac principal coordinates analysis based on 12 taxa separated most cancers from other samples with greatest separation of node positive cases. These studies begin to develop a framework for exploiting the oral microbiome for monitoring oral cancer development, progression and recurrence. Copyright:

Original languageEnglish (US)
Article numbere98741
JournalPLoS One
Volume9
Issue number6
DOIs
StatePublished - Jun 2 2014

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Mouth Neoplasms
Microbiota
Ribosomal DNA
mouth
Bacteria
Tissue
neoplasms
Monitoring
Chemical analysis
Neoplasms
Rothia (bacteria)
Mouth Floor
sampling
Firmicutes
Actinobacteria
Streptococcus
tongue
Tongue
human diseases
microbiome

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Changes in abundance of oral microbiota associated with oral cancer. / Schmidt, Brian; Kuczynski, Justin; Bhattacharya, Aditi; Huey, Bing; Corby, Patricia M.; Queiroz, Erica L S; Nightingale, Kira; Kerr, A. Ross; DeLacure, Mark D.; Veeramachaneni, Ratna; Olshen, Adam B.; Albertson, Donna.

In: PLoS One, Vol. 9, No. 6, e98741, 02.06.2014.

Research output: Contribution to journalArticle

Schmidt, B, Kuczynski, J, Bhattacharya, A, Huey, B, Corby, PM, Queiroz, ELS, Nightingale, K, Kerr, AR, DeLacure, MD, Veeramachaneni, R, Olshen, AB & Albertson, D 2014, 'Changes in abundance of oral microbiota associated with oral cancer', PLoS One, vol. 9, no. 6, e98741. https://doi.org/10.1371/journal.pone.0098741
Schmidt, Brian ; Kuczynski, Justin ; Bhattacharya, Aditi ; Huey, Bing ; Corby, Patricia M. ; Queiroz, Erica L S ; Nightingale, Kira ; Kerr, A. Ross ; DeLacure, Mark D. ; Veeramachaneni, Ratna ; Olshen, Adam B. ; Albertson, Donna. / Changes in abundance of oral microbiota associated with oral cancer. In: PLoS One. 2014 ; Vol. 9, No. 6.
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