Cerebellum- and forebrain-derived stem cells possess intrinsic regional character

Corinna Klein, Simon J.B. Butt, Robert P. Machold, Jane E. Johnson, Gordon Fishell

Research output: Contribution to journalArticle

Abstract

The existence of stem cells in the adult nervous system is well recognized; however, the potential of these cells is still widely debated. We demonstrate that neural stem cells exist within the embryonic and adult cerebellum. Comparing the potential of neural stem cells derived from the forebrain and cerebellum, we find that progeny derived from each of these brain regions retain regional character in vitro as well as after homotopic transplantation. However, when ectopically transplanted, neurosphere-derived cells from either region are largely unable to generate neurons. With regard specifically to embryonic and adult cerebellar stem cells, we observe that they are able to give rise to neurons that resemble different select classes of cerebellar subclasses when grafted into the perinatal host cerebellum. Most notably, upon transplantation to the perinatal cerebellum, cerebellar stem cells from all ages are able to acquire the position and mature electrophysiological properties of cerebellar granule cells.

Original languageEnglish (US)
Pages (from-to)4497-4508
Number of pages12
JournalDevelopment
Volume132
Issue number20
DOIs
StatePublished - Oct 1 2005

Fingerprint

Prosencephalon
Cerebellum
Stem Cells
Adult Stem Cells
Neural Stem Cells
Transplantation
Neurons
Nervous System
Brain

Keywords

  • Cerebellum
  • Forebrain
  • Mouse
  • Neural stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Cerebellum- and forebrain-derived stem cells possess intrinsic regional character. / Klein, Corinna; Butt, Simon J.B.; Machold, Robert P.; Johnson, Jane E.; Fishell, Gordon.

In: Development, Vol. 132, No. 20, 01.10.2005, p. 4497-4508.

Research output: Contribution to journalArticle

Klein, Corinna ; Butt, Simon J.B. ; Machold, Robert P. ; Johnson, Jane E. ; Fishell, Gordon. / Cerebellum- and forebrain-derived stem cells possess intrinsic regional character. In: Development. 2005 ; Vol. 132, No. 20. pp. 4497-4508.
@article{1c2d619a2385477bb1a08951f006dd4f,
title = "Cerebellum- and forebrain-derived stem cells possess intrinsic regional character",
abstract = "The existence of stem cells in the adult nervous system is well recognized; however, the potential of these cells is still widely debated. We demonstrate that neural stem cells exist within the embryonic and adult cerebellum. Comparing the potential of neural stem cells derived from the forebrain and cerebellum, we find that progeny derived from each of these brain regions retain regional character in vitro as well as after homotopic transplantation. However, when ectopically transplanted, neurosphere-derived cells from either region are largely unable to generate neurons. With regard specifically to embryonic and adult cerebellar stem cells, we observe that they are able to give rise to neurons that resemble different select classes of cerebellar subclasses when grafted into the perinatal host cerebellum. Most notably, upon transplantation to the perinatal cerebellum, cerebellar stem cells from all ages are able to acquire the position and mature electrophysiological properties of cerebellar granule cells.",
keywords = "Cerebellum, Forebrain, Mouse, Neural stem cell",
author = "Corinna Klein and Butt, {Simon J.B.} and Machold, {Robert P.} and Johnson, {Jane E.} and Gordon Fishell",
year = "2005",
month = "10",
day = "1",
doi = "10.1242/dev.02037",
language = "English (US)",
volume = "132",
pages = "4497--4508",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "20",

}

TY - JOUR

T1 - Cerebellum- and forebrain-derived stem cells possess intrinsic regional character

AU - Klein, Corinna

AU - Butt, Simon J.B.

AU - Machold, Robert P.

AU - Johnson, Jane E.

AU - Fishell, Gordon

PY - 2005/10/1

Y1 - 2005/10/1

N2 - The existence of stem cells in the adult nervous system is well recognized; however, the potential of these cells is still widely debated. We demonstrate that neural stem cells exist within the embryonic and adult cerebellum. Comparing the potential of neural stem cells derived from the forebrain and cerebellum, we find that progeny derived from each of these brain regions retain regional character in vitro as well as after homotopic transplantation. However, when ectopically transplanted, neurosphere-derived cells from either region are largely unable to generate neurons. With regard specifically to embryonic and adult cerebellar stem cells, we observe that they are able to give rise to neurons that resemble different select classes of cerebellar subclasses when grafted into the perinatal host cerebellum. Most notably, upon transplantation to the perinatal cerebellum, cerebellar stem cells from all ages are able to acquire the position and mature electrophysiological properties of cerebellar granule cells.

AB - The existence of stem cells in the adult nervous system is well recognized; however, the potential of these cells is still widely debated. We demonstrate that neural stem cells exist within the embryonic and adult cerebellum. Comparing the potential of neural stem cells derived from the forebrain and cerebellum, we find that progeny derived from each of these brain regions retain regional character in vitro as well as after homotopic transplantation. However, when ectopically transplanted, neurosphere-derived cells from either region are largely unable to generate neurons. With regard specifically to embryonic and adult cerebellar stem cells, we observe that they are able to give rise to neurons that resemble different select classes of cerebellar subclasses when grafted into the perinatal host cerebellum. Most notably, upon transplantation to the perinatal cerebellum, cerebellar stem cells from all ages are able to acquire the position and mature electrophysiological properties of cerebellar granule cells.

KW - Cerebellum

KW - Forebrain

KW - Mouse

KW - Neural stem cell

UR - http://www.scopus.com/inward/record.url?scp=27744521637&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27744521637&partnerID=8YFLogxK

U2 - 10.1242/dev.02037

DO - 10.1242/dev.02037

M3 - Article

C2 - 16162650

AN - SCOPUS:27744521637

VL - 132

SP - 4497

EP - 4508

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 20

ER -