Centering of a radial microtubule array by translocation along microtubules spontaneously nucleated in the cytoplasm

Viacheslav Malikov, Eric N. Cytrynbaum, Anna Kashina, Alexander Mogilner, Vladimir Rodionov

Research output: Contribution to journalArticle

Abstract

Positioning of a radial array of microtubules (MTs) in the cell centre is crucial for cytoplasmic organization, but the mechanisms of such centering are difficult to study in intact cells that have pre-formed radial arrays. Here, we use cytoplasmic fragments of melanophores, and cytoplasts of BS-C-1 cells to study MT centering mechanisms. Using live imaging and computer modelling, we show that the MT aster finds a central location in the cytoplasm by moving along spontaneously nucleated non-astral MTs towards a point at which MT nucleation events occur equally on all sides. We hypothesize that similar mechanisms, in the presence of the centrosome, contribute to this centering mechanism and ensure the robustness of cytoplasmic organization.

Original languageEnglish (US)
Pages (from-to)1113-1118
Number of pages6
JournalNature Cell Biology
Volume7
Issue number12
DOIs
StatePublished - Dec 2005

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Microtubules
Cytoplasm
Melanophores
Centrosome

ASJC Scopus subject areas

  • Cell Biology

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Centering of a radial microtubule array by translocation along microtubules spontaneously nucleated in the cytoplasm. / Malikov, Viacheslav; Cytrynbaum, Eric N.; Kashina, Anna; Mogilner, Alexander; Rodionov, Vladimir.

In: Nature Cell Biology, Vol. 7, No. 12, 12.2005, p. 1113-1118.

Research output: Contribution to journalArticle

Malikov, Viacheslav ; Cytrynbaum, Eric N. ; Kashina, Anna ; Mogilner, Alexander ; Rodionov, Vladimir. / Centering of a radial microtubule array by translocation along microtubules spontaneously nucleated in the cytoplasm. In: Nature Cell Biology. 2005 ; Vol. 7, No. 12. pp. 1113-1118.
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