Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates

Jason M. Belitsky, Stephanie J. Leslie, Paramjit S. Arora, Terry A. Beerman, Peter B. Dervan

Research output: Contribution to journalArticle

Abstract

The cellular uptake and localization properties of DNA binding N-methylpyrrole/N-methylimidazole polyamide dye conjugates in a variety of living ells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, polyamide dye conjugates localize mainly in the cytoplasm and not in the nucleus. Reagents such as methanol typically used to fix cells for microscopy significantly alter the cellular localization of these DNA-binding ligands.

Original languageEnglish (US)
Pages (from-to)3313-3318
Number of pages6
JournalBioorganic and Medicinal Chemistry
Volume10
Issue number10
DOIs
StatePublished - Aug 19 2002

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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