Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates

Jason M. Belitsky, Stephanie J. Leslie, Paramjit Arora, Terry A. Beerman, Peter B. Dervan

Research output: Contribution to journalArticle

Abstract

The cellular uptake and localization properties of DNA binding N-methylpyrrole/N-methylimidazole polyamide dye conjugates in a variety of living ells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, polyamide dye conjugates localize mainly in the cytoplasm and not in the nucleus. Reagents such as methanol typically used to fix cells for microscopy significantly alter the cellular localization of these DNA-binding ligands.

Original languageEnglish (US)
Pages (from-to)3313-3318
Number of pages6
JournalBioorganic and Medicinal Chemistry
Volume10
Issue number10
DOIs
StatePublished - 2002

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Nylons
Microscopic examination
Coloring Agents
T-cells
DNA
Confocal Microscopy
Methanol
Microscopy
Cytoplasm
Ligands
Scanning
T-Lymphocytes
Cell Line
Lasers
1-methylimidazole
N-methylpyrrole

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates. / Belitsky, Jason M.; Leslie, Stephanie J.; Arora, Paramjit; Beerman, Terry A.; Dervan, Peter B.

In: Bioorganic and Medicinal Chemistry, Vol. 10, No. 10, 2002, p. 3313-3318.

Research output: Contribution to journalArticle

Belitsky, Jason M. ; Leslie, Stephanie J. ; Arora, Paramjit ; Beerman, Terry A. ; Dervan, Peter B. / Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates. In: Bioorganic and Medicinal Chemistry. 2002 ; Vol. 10, No. 10. pp. 3313-3318.
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