Abstract
Activation of 5-HT(1A) receptors produces many different physiologic responses, which may be due to their localization on diverse cells in the brain. A 5-HT(1A) receptor antipeptide (aa170-186) antibody was produced that showed both high titer for peptide binding and immunocytochemical staining. Studies performed in perfusion-fixed brain tissue showed immunoreactive neurons, glial, and ependymal cells in the rat, mouse, cat, and monkey. Results from our studies of Macaca vesicularis brains are presented. We observed two main neuronal labeling patterns in the primate brain: (1) A general, diffuse somatodendritic distribution of 5-HT(1A) receptor immunoreactivity is seen in the raphe nuclei where the dendritic shaft, its branches and spines, and the entire perikaryon are immunolabeled. This pattern is also observed in the nucleus locus coeruleus, in scattered large brainstem reticular neurons, and in dentate gyrus hilar interneurons. (2) A discrete localization of 5-HT(1A) receptor immunoreactivity on the initial axon segment (axon hillock) is noted in pyramidal neurons of layer III and V of cerebral cortex, Cornu Ammonus (1-4) of the hippocampus, and in most brainstem and cervical spinal cord motoneurons. In addition to neuronal labeling, 5-HT(1A) receptor immunoreactivity is seen in the cell body and processes of astrocytes, and other nonneuronal cells. This pattern is particularly evident in the white matter of cerebral cortex and spinal cord, the pontine nuclei, the brainstem tectum, and the hilus of the dentate gyrus. The clinical implications of 5-HT(1A) cellular localization are briefly discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 35-46 |
| Number of pages | 12 |
| Journal | Neuropsychopharmacology |
| Volume | 14 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1996 |
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Keywords
- Astrocytes
- Autoreceptor
- Axon hillock
- Cerebral cortex
- Dentate gyrus
- Dorsal raphe nucleus
- Hippocampus
- Immunocytochemistry
- Locus coeruleus
- Monkey
ASJC Scopus subject areas
- Pharmacology
Cite this
Cellular localization of the 5-HT(1A) receptor in primate brain neurons and glial cells. / Azmitia, E. C.; Gannon, P. J.; Kheck, N. M.; Whitaker-Azmitia, P. M.
In: Neuropsychopharmacology, Vol. 14, No. 1, 1996, p. 35-46.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cellular localization of the 5-HT(1A) receptor in primate brain neurons and glial cells
AU - Azmitia, E. C.
AU - Gannon, P. J.
AU - Kheck, N. M.
AU - Whitaker-Azmitia, P. M.
PY - 1996
Y1 - 1996
N2 - Activation of 5-HT(1A) receptors produces many different physiologic responses, which may be due to their localization on diverse cells in the brain. A 5-HT(1A) receptor antipeptide (aa170-186) antibody was produced that showed both high titer for peptide binding and immunocytochemical staining. Studies performed in perfusion-fixed brain tissue showed immunoreactive neurons, glial, and ependymal cells in the rat, mouse, cat, and monkey. Results from our studies of Macaca vesicularis brains are presented. We observed two main neuronal labeling patterns in the primate brain: (1) A general, diffuse somatodendritic distribution of 5-HT(1A) receptor immunoreactivity is seen in the raphe nuclei where the dendritic shaft, its branches and spines, and the entire perikaryon are immunolabeled. This pattern is also observed in the nucleus locus coeruleus, in scattered large brainstem reticular neurons, and in dentate gyrus hilar interneurons. (2) A discrete localization of 5-HT(1A) receptor immunoreactivity on the initial axon segment (axon hillock) is noted in pyramidal neurons of layer III and V of cerebral cortex, Cornu Ammonus (1-4) of the hippocampus, and in most brainstem and cervical spinal cord motoneurons. In addition to neuronal labeling, 5-HT(1A) receptor immunoreactivity is seen in the cell body and processes of astrocytes, and other nonneuronal cells. This pattern is particularly evident in the white matter of cerebral cortex and spinal cord, the pontine nuclei, the brainstem tectum, and the hilus of the dentate gyrus. The clinical implications of 5-HT(1A) cellular localization are briefly discussed.
AB - Activation of 5-HT(1A) receptors produces many different physiologic responses, which may be due to their localization on diverse cells in the brain. A 5-HT(1A) receptor antipeptide (aa170-186) antibody was produced that showed both high titer for peptide binding and immunocytochemical staining. Studies performed in perfusion-fixed brain tissue showed immunoreactive neurons, glial, and ependymal cells in the rat, mouse, cat, and monkey. Results from our studies of Macaca vesicularis brains are presented. We observed two main neuronal labeling patterns in the primate brain: (1) A general, diffuse somatodendritic distribution of 5-HT(1A) receptor immunoreactivity is seen in the raphe nuclei where the dendritic shaft, its branches and spines, and the entire perikaryon are immunolabeled. This pattern is also observed in the nucleus locus coeruleus, in scattered large brainstem reticular neurons, and in dentate gyrus hilar interneurons. (2) A discrete localization of 5-HT(1A) receptor immunoreactivity on the initial axon segment (axon hillock) is noted in pyramidal neurons of layer III and V of cerebral cortex, Cornu Ammonus (1-4) of the hippocampus, and in most brainstem and cervical spinal cord motoneurons. In addition to neuronal labeling, 5-HT(1A) receptor immunoreactivity is seen in the cell body and processes of astrocytes, and other nonneuronal cells. This pattern is particularly evident in the white matter of cerebral cortex and spinal cord, the pontine nuclei, the brainstem tectum, and the hilus of the dentate gyrus. The clinical implications of 5-HT(1A) cellular localization are briefly discussed.
KW - Astrocytes
KW - Autoreceptor
KW - Axon hillock
KW - Cerebral cortex
KW - Dentate gyrus
KW - Dorsal raphe nucleus
KW - Hippocampus
KW - Immunocytochemistry
KW - Locus coeruleus
KW - Monkey
UR - http://www.scopus.com/inward/record.url?scp=0030023608&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030023608&partnerID=8YFLogxK
U2 - 10.1016/S0893-133X(96)80057-1
DO - 10.1016/S0893-133X(96)80057-1
M3 - Article
C2 - 8719028
AN - SCOPUS:0030023608
VL - 14
SP - 35
EP - 46
JO - Neuropsychopharmacology
JF - Neuropsychopharmacology
SN - 0893-133X
IS - 1
ER -