Cellular localization of the 5-HT(1A) receptor in primate brain neurons and glial cells

E. C. Azmitia, P. J. Gannon, N. M. Kheck, P. M. Whitaker-Azmitia

Research output: Contribution to journalArticle

Abstract

Activation of 5-HT(1A) receptors produces many different physiologic responses, which may be due to their localization on diverse cells in the brain. A 5-HT(1A) receptor antipeptide (aa170-186) antibody was produced that showed both high titer for peptide binding and immunocytochemical staining. Studies performed in perfusion-fixed brain tissue showed immunoreactive neurons, glial, and ependymal cells in the rat, mouse, cat, and monkey. Results from our studies of Macaca vesicularis brains are presented. We observed two main neuronal labeling patterns in the primate brain: (1) A general, diffuse somatodendritic distribution of 5-HT(1A) receptor immunoreactivity is seen in the raphe nuclei where the dendritic shaft, its branches and spines, and the entire perikaryon are immunolabeled. This pattern is also observed in the nucleus locus coeruleus, in scattered large brainstem reticular neurons, and in dentate gyrus hilar interneurons. (2) A discrete localization of 5-HT(1A) receptor immunoreactivity on the initial axon segment (axon hillock) is noted in pyramidal neurons of layer III and V of cerebral cortex, Cornu Ammonus (1-4) of the hippocampus, and in most brainstem and cervical spinal cord motoneurons. In addition to neuronal labeling, 5-HT(1A) receptor immunoreactivity is seen in the cell body and processes of astrocytes, and other nonneuronal cells. This pattern is particularly evident in the white matter of cerebral cortex and spinal cord, the pontine nuclei, the brainstem tectum, and the hilus of the dentate gyrus. The clinical implications of 5-HT(1A) cellular localization are briefly discussed.

Original languageEnglish (US)
Pages (from-to)35-46
Number of pages12
JournalNeuropsychopharmacology
Volume14
Issue number1
DOIs
StatePublished - 1996

Fingerprint

Receptor, Serotonin, 5-HT1A
Neuroglia
Primates
Neurons
Brain
Brain Stem
Dentate Gyrus
Cerebral Cortex
Raphe Nuclei
Locus Coeruleus
Pyramidal Cells
Macaca
Motor Neurons
Interneurons
Astrocytes
Haplorhini
Hippocampus
Serotonin
Spinal Cord
Cats

Keywords

  • Astrocytes
  • Autoreceptor
  • Axon hillock
  • Cerebral cortex
  • Dentate gyrus
  • Dorsal raphe nucleus
  • Hippocampus
  • Immunocytochemistry
  • Locus coeruleus
  • Monkey

ASJC Scopus subject areas

  • Pharmacology

Cite this

Cellular localization of the 5-HT(1A) receptor in primate brain neurons and glial cells. / Azmitia, E. C.; Gannon, P. J.; Kheck, N. M.; Whitaker-Azmitia, P. M.

In: Neuropsychopharmacology, Vol. 14, No. 1, 1996, p. 35-46.

Research output: Contribution to journalArticle

Azmitia, E. C. ; Gannon, P. J. ; Kheck, N. M. ; Whitaker-Azmitia, P. M. / Cellular localization of the 5-HT(1A) receptor in primate brain neurons and glial cells. In: Neuropsychopharmacology. 1996 ; Vol. 14, No. 1. pp. 35-46.
@article{2e71615f8946419d9835dafdea3710a5,
title = "Cellular localization of the 5-HT(1A) receptor in primate brain neurons and glial cells",
abstract = "Activation of 5-HT(1A) receptors produces many different physiologic responses, which may be due to their localization on diverse cells in the brain. A 5-HT(1A) receptor antipeptide (aa170-186) antibody was produced that showed both high titer for peptide binding and immunocytochemical staining. Studies performed in perfusion-fixed brain tissue showed immunoreactive neurons, glial, and ependymal cells in the rat, mouse, cat, and monkey. Results from our studies of Macaca vesicularis brains are presented. We observed two main neuronal labeling patterns in the primate brain: (1) A general, diffuse somatodendritic distribution of 5-HT(1A) receptor immunoreactivity is seen in the raphe nuclei where the dendritic shaft, its branches and spines, and the entire perikaryon are immunolabeled. This pattern is also observed in the nucleus locus coeruleus, in scattered large brainstem reticular neurons, and in dentate gyrus hilar interneurons. (2) A discrete localization of 5-HT(1A) receptor immunoreactivity on the initial axon segment (axon hillock) is noted in pyramidal neurons of layer III and V of cerebral cortex, Cornu Ammonus (1-4) of the hippocampus, and in most brainstem and cervical spinal cord motoneurons. In addition to neuronal labeling, 5-HT(1A) receptor immunoreactivity is seen in the cell body and processes of astrocytes, and other nonneuronal cells. This pattern is particularly evident in the white matter of cerebral cortex and spinal cord, the pontine nuclei, the brainstem tectum, and the hilus of the dentate gyrus. The clinical implications of 5-HT(1A) cellular localization are briefly discussed.",
keywords = "Astrocytes, Autoreceptor, Axon hillock, Cerebral cortex, Dentate gyrus, Dorsal raphe nucleus, Hippocampus, Immunocytochemistry, Locus coeruleus, Monkey",
author = "Azmitia, {E. C.} and Gannon, {P. J.} and Kheck, {N. M.} and Whitaker-Azmitia, {P. M.}",
year = "1996",
doi = "10.1016/S0893-133X(96)80057-1",
language = "English (US)",
volume = "14",
pages = "35--46",
journal = "Neuropsychopharmacology",
issn = "0893-133X",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Cellular localization of the 5-HT(1A) receptor in primate brain neurons and glial cells

AU - Azmitia, E. C.

AU - Gannon, P. J.

AU - Kheck, N. M.

AU - Whitaker-Azmitia, P. M.

PY - 1996

Y1 - 1996

N2 - Activation of 5-HT(1A) receptors produces many different physiologic responses, which may be due to their localization on diverse cells in the brain. A 5-HT(1A) receptor antipeptide (aa170-186) antibody was produced that showed both high titer for peptide binding and immunocytochemical staining. Studies performed in perfusion-fixed brain tissue showed immunoreactive neurons, glial, and ependymal cells in the rat, mouse, cat, and monkey. Results from our studies of Macaca vesicularis brains are presented. We observed two main neuronal labeling patterns in the primate brain: (1) A general, diffuse somatodendritic distribution of 5-HT(1A) receptor immunoreactivity is seen in the raphe nuclei where the dendritic shaft, its branches and spines, and the entire perikaryon are immunolabeled. This pattern is also observed in the nucleus locus coeruleus, in scattered large brainstem reticular neurons, and in dentate gyrus hilar interneurons. (2) A discrete localization of 5-HT(1A) receptor immunoreactivity on the initial axon segment (axon hillock) is noted in pyramidal neurons of layer III and V of cerebral cortex, Cornu Ammonus (1-4) of the hippocampus, and in most brainstem and cervical spinal cord motoneurons. In addition to neuronal labeling, 5-HT(1A) receptor immunoreactivity is seen in the cell body and processes of astrocytes, and other nonneuronal cells. This pattern is particularly evident in the white matter of cerebral cortex and spinal cord, the pontine nuclei, the brainstem tectum, and the hilus of the dentate gyrus. The clinical implications of 5-HT(1A) cellular localization are briefly discussed.

AB - Activation of 5-HT(1A) receptors produces many different physiologic responses, which may be due to their localization on diverse cells in the brain. A 5-HT(1A) receptor antipeptide (aa170-186) antibody was produced that showed both high titer for peptide binding and immunocytochemical staining. Studies performed in perfusion-fixed brain tissue showed immunoreactive neurons, glial, and ependymal cells in the rat, mouse, cat, and monkey. Results from our studies of Macaca vesicularis brains are presented. We observed two main neuronal labeling patterns in the primate brain: (1) A general, diffuse somatodendritic distribution of 5-HT(1A) receptor immunoreactivity is seen in the raphe nuclei where the dendritic shaft, its branches and spines, and the entire perikaryon are immunolabeled. This pattern is also observed in the nucleus locus coeruleus, in scattered large brainstem reticular neurons, and in dentate gyrus hilar interneurons. (2) A discrete localization of 5-HT(1A) receptor immunoreactivity on the initial axon segment (axon hillock) is noted in pyramidal neurons of layer III and V of cerebral cortex, Cornu Ammonus (1-4) of the hippocampus, and in most brainstem and cervical spinal cord motoneurons. In addition to neuronal labeling, 5-HT(1A) receptor immunoreactivity is seen in the cell body and processes of astrocytes, and other nonneuronal cells. This pattern is particularly evident in the white matter of cerebral cortex and spinal cord, the pontine nuclei, the brainstem tectum, and the hilus of the dentate gyrus. The clinical implications of 5-HT(1A) cellular localization are briefly discussed.

KW - Astrocytes

KW - Autoreceptor

KW - Axon hillock

KW - Cerebral cortex

KW - Dentate gyrus

KW - Dorsal raphe nucleus

KW - Hippocampus

KW - Immunocytochemistry

KW - Locus coeruleus

KW - Monkey

UR - http://www.scopus.com/inward/record.url?scp=0030023608&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030023608&partnerID=8YFLogxK

U2 - 10.1016/S0893-133X(96)80057-1

DO - 10.1016/S0893-133X(96)80057-1

M3 - Article

VL - 14

SP - 35

EP - 46

JO - Neuropsychopharmacology

JF - Neuropsychopharmacology

SN - 0893-133X

IS - 1

ER -