Cellular localization of Arabidopsis xyloglucan endotransglycosylase-related proteins during development and after wind stimulation

D. M. Antosiewicz, M. M. Purugganan, D. H. Polisensky, J. Braam

Research output: Contribution to journalArticle

Abstract

A gene family encoding xyloglucan endotransglycosylase (XET)-related proteins exists in Arabidopsis. TCH4, a member of this family, is strongly up-regulated by environmental stimuli and encodes an XET capable of modifying cell wall xyloglucans. To investigate XET localization we generated antibodies against the TCH4 carboxyl terminus. The antibodies recognized TCH4 and possibly other XET-related proteins. These data indicate that XETs accumulate in expanding cell, at the sites of intercellular airspace formation, and at the bases of leaves, cotyledons, and hypocotyls. XETs also accumulated in vascular tissue, where cell wall modifications lead to the formation of tracheary elements and sieve tubes. Thus, XETs may function in modifying cell walls to allow growth, airspace formation, the development of vasculature, and reinforcement of regions under mechanical strain. Following wind stimulation, overall XET levels appeared to decrease in the leaves of wind-stimulated plants. However, consistent with an increase in TCH4 mRNA levels following wind, there were regions that showed increased immunoreaction, including sites around cells of the pith parenchyma, between the vascular elements, and within the epidermis. These results indicate that TCH4 may contribute to the adaptive changes in morphogenesis that occur in Arabidopsis following exposure to mechanical stimuli.
Original languageUndefined
Pages (from-to)1319-28
JournalPlant Physiology
Volume115
Issue number4
StatePublished - 1997

Keywords

  • Arabidopsis/genetics/*physiology *Arabidopsis Proteins Cell Wall/metabolism Cotyledon Gene Expression Regulation, Developmental *Gene Expression Regulation, Plant Genes, Plant Glycosyltransferases/*biosynthesis Hypocotyl Multigene Family Physical Stimulation Plant Leaves Plant Proteins/biosynthesis Wind

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Cellular localization of Arabidopsis xyloglucan endotransglycosylase-related proteins during development and after wind stimulation. / Antosiewicz, D. M.; Purugganan, M. M.; Polisensky, D. H.; Braam, J.

In: Plant Physiology, Vol. 115, No. 4, 1997, p. 1319-28.

Research output: Contribution to journalArticle

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abstract = "A gene family encoding xyloglucan endotransglycosylase (XET)-related proteins exists in Arabidopsis. TCH4, a member of this family, is strongly up-regulated by environmental stimuli and encodes an XET capable of modifying cell wall xyloglucans. To investigate XET localization we generated antibodies against the TCH4 carboxyl terminus. The antibodies recognized TCH4 and possibly other XET-related proteins. These data indicate that XETs accumulate in expanding cell, at the sites of intercellular airspace formation, and at the bases of leaves, cotyledons, and hypocotyls. XETs also accumulated in vascular tissue, where cell wall modifications lead to the formation of tracheary elements and sieve tubes. Thus, XETs may function in modifying cell walls to allow growth, airspace formation, the development of vasculature, and reinforcement of regions under mechanical strain. Following wind stimulation, overall XET levels appeared to decrease in the leaves of wind-stimulated plants. However, consistent with an increase in TCH4 mRNA levels following wind, there were regions that showed increased immunoreaction, including sites around cells of the pith parenchyma, between the vascular elements, and within the epidermis. These results indicate that TCH4 may contribute to the adaptive changes in morphogenesis that occur in Arabidopsis following exposure to mechanical stimuli.",
keywords = "Arabidopsis/genetics/*physiology *Arabidopsis Proteins Cell Wall/metabolism Cotyledon Gene Expression Regulation, Developmental *Gene Expression Regulation, Plant Genes, Plant Glycosyltransferases/*biosynthesis Hypocotyl Multigene Family Physical Stimulation Plant Leaves Plant Proteins/biosynthesis Wind",
author = "Antosiewicz, {D. M.} and Purugganan, {M. M.} and Polisensky, {D. H.} and J. Braam",
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AU - Antosiewicz, D. M.

AU - Purugganan, M. M.

AU - Polisensky, D. H.

AU - Braam, J.

N1 - Antosiewicz, D M Purugganan, M M Polisensky, D H Braam, J GM07833/GM/NIGMS NIH HHS/United States Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. United states Plant Physiol. 1997 Dec;115(4):1319-28.

PY - 1997

Y1 - 1997

N2 - A gene family encoding xyloglucan endotransglycosylase (XET)-related proteins exists in Arabidopsis. TCH4, a member of this family, is strongly up-regulated by environmental stimuli and encodes an XET capable of modifying cell wall xyloglucans. To investigate XET localization we generated antibodies against the TCH4 carboxyl terminus. The antibodies recognized TCH4 and possibly other XET-related proteins. These data indicate that XETs accumulate in expanding cell, at the sites of intercellular airspace formation, and at the bases of leaves, cotyledons, and hypocotyls. XETs also accumulated in vascular tissue, where cell wall modifications lead to the formation of tracheary elements and sieve tubes. Thus, XETs may function in modifying cell walls to allow growth, airspace formation, the development of vasculature, and reinforcement of regions under mechanical strain. Following wind stimulation, overall XET levels appeared to decrease in the leaves of wind-stimulated plants. However, consistent with an increase in TCH4 mRNA levels following wind, there were regions that showed increased immunoreaction, including sites around cells of the pith parenchyma, between the vascular elements, and within the epidermis. These results indicate that TCH4 may contribute to the adaptive changes in morphogenesis that occur in Arabidopsis following exposure to mechanical stimuli.

AB - A gene family encoding xyloglucan endotransglycosylase (XET)-related proteins exists in Arabidopsis. TCH4, a member of this family, is strongly up-regulated by environmental stimuli and encodes an XET capable of modifying cell wall xyloglucans. To investigate XET localization we generated antibodies against the TCH4 carboxyl terminus. The antibodies recognized TCH4 and possibly other XET-related proteins. These data indicate that XETs accumulate in expanding cell, at the sites of intercellular airspace formation, and at the bases of leaves, cotyledons, and hypocotyls. XETs also accumulated in vascular tissue, where cell wall modifications lead to the formation of tracheary elements and sieve tubes. Thus, XETs may function in modifying cell walls to allow growth, airspace formation, the development of vasculature, and reinforcement of regions under mechanical strain. Following wind stimulation, overall XET levels appeared to decrease in the leaves of wind-stimulated plants. However, consistent with an increase in TCH4 mRNA levels following wind, there were regions that showed increased immunoreaction, including sites around cells of the pith parenchyma, between the vascular elements, and within the epidermis. These results indicate that TCH4 may contribute to the adaptive changes in morphogenesis that occur in Arabidopsis following exposure to mechanical stimuli.

KW - Arabidopsis/genetics/physiology Arabidopsis Proteins Cell Wall/metabolism Cotyledon Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant Glycosyltransferases/biosynthesis Hypocotyl Multigene Family Physical Stimulation

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JF - Plant Physiology

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ER -