Cells in the posterior thalamus project to both amygdala and temporal cortex: A quantitative retrograde double-labeling study in the rat

Neot N. Doron, Joseph Ledoux

Research output: Contribution to journalArticle

Abstract

Auditory information from the posterior thalamus reaches the lateral nucleus of the amygdala (LA) by way of two pathways: A direct thalamo-amygdala projection and a polysynaptic thalamo-cortico-amygdala projection. However, the quantitative extent of thalamic neurons that project to the LA or to the auditory association cortex (AAC) is not known. Furthermore, the extent and topographical distribution of bifurcating cells that project to both LA and AAC are also unknown. Therefore, separate tracers were injected into LA and either into all of AAC or within discrete regions of AAC, such as temporal areas TE3 or perirhinal cortex (PRh), and quantitative analyses were performed on labeling within the subregions of the auditory thalamus in rats. Following LA injections, retrogradely labeled cells were most numerous in the posterior intralaminar nucleus (PIN; 48.0% of all labeled thalamic cells), whereas labeled cells following injections of the entire AAC were most numerous in the dorsal division of the medial geniculate nucleus (MGd; 32.9% of all labeled thalamic cells). Following AAC injections localized to only TE3, the MGd again had the majority of labeled cells (35.9%), whereas following AAC injections localized to PRh, the PIN had the most labeled cells (32.8%). Double-labeled cells were found in all the thalamic regions studied and were most commonly observed in the PIN (43.7% of all double-labeled cells following injections into LA and throughout the AAC). The percentage of double-labeled cells as a proportion of either LA-projecting or AAC-projecting cells varied among the thalamic nuclei studied, ranging from 2.9% up to 42.4%. The topographic distribution of double-labeled cells in the thalamic nuclei resembled that of single-labeled cells following LA injections more than single-labeled cells following AAC injection. These findings suggest that double-labeled cells contribute substantially to many of the direct thalamo-amygdala and indirect thalamo-AAC-amygdala projections. Among other functions, these bifurcating cells may help regulate the processing of input to the LA arriving from these two pathways to allow for certain types of plasticity in the LA during fear conditioning. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)257-274
Number of pages18
JournalJournal of Comparative Neurology
Volume425
Issue number2
DOIs
StatePublished - Sep 18 2000

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Temporal Lobe
Amygdala
Thalamus
Auditory Cortex
Injections
Thalamic Nuclei
Geniculate Bodies
Fear

Keywords

  • Auditory cortex
  • Fear conditioning
  • Lateral posterior nucleus
  • Medial geniculate nucleus
  • Perirhinal cortex
  • Posterior intralaminar nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Cells in the posterior thalamus project to both amygdala and temporal cortex: A quantitative retrograde double-labeling study in the rat",
abstract = "Auditory information from the posterior thalamus reaches the lateral nucleus of the amygdala (LA) by way of two pathways: A direct thalamo-amygdala projection and a polysynaptic thalamo-cortico-amygdala projection. However, the quantitative extent of thalamic neurons that project to the LA or to the auditory association cortex (AAC) is not known. Furthermore, the extent and topographical distribution of bifurcating cells that project to both LA and AAC are also unknown. Therefore, separate tracers were injected into LA and either into all of AAC or within discrete regions of AAC, such as temporal areas TE3 or perirhinal cortex (PRh), and quantitative analyses were performed on labeling within the subregions of the auditory thalamus in rats. Following LA injections, retrogradely labeled cells were most numerous in the posterior intralaminar nucleus (PIN; 48.0{\%} of all labeled thalamic cells), whereas labeled cells following injections of the entire AAC were most numerous in the dorsal division of the medial geniculate nucleus (MGd; 32.9{\%} of all labeled thalamic cells). Following AAC injections localized to only TE3, the MGd again had the majority of labeled cells (35.9{\%}), whereas following AAC injections localized to PRh, the PIN had the most labeled cells (32.8{\%}). Double-labeled cells were found in all the thalamic regions studied and were most commonly observed in the PIN (43.7{\%} of all double-labeled cells following injections into LA and throughout the AAC). The percentage of double-labeled cells as a proportion of either LA-projecting or AAC-projecting cells varied among the thalamic nuclei studied, ranging from 2.9{\%} up to 42.4{\%}. The topographic distribution of double-labeled cells in the thalamic nuclei resembled that of single-labeled cells following LA injections more than single-labeled cells following AAC injection. These findings suggest that double-labeled cells contribute substantially to many of the direct thalamo-amygdala and indirect thalamo-AAC-amygdala projections. Among other functions, these bifurcating cells may help regulate the processing of input to the LA arriving from these two pathways to allow for certain types of plasticity in the LA during fear conditioning. (C) 2000 Wiley-Liss, Inc.",
keywords = "Auditory cortex, Fear conditioning, Lateral posterior nucleus, Medial geniculate nucleus, Perirhinal cortex, Posterior intralaminar nucleus",
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T2 - A quantitative retrograde double-labeling study in the rat

AU - Doron, Neot N.

AU - Ledoux, Joseph

PY - 2000/9/18

Y1 - 2000/9/18

N2 - Auditory information from the posterior thalamus reaches the lateral nucleus of the amygdala (LA) by way of two pathways: A direct thalamo-amygdala projection and a polysynaptic thalamo-cortico-amygdala projection. However, the quantitative extent of thalamic neurons that project to the LA or to the auditory association cortex (AAC) is not known. Furthermore, the extent and topographical distribution of bifurcating cells that project to both LA and AAC are also unknown. Therefore, separate tracers were injected into LA and either into all of AAC or within discrete regions of AAC, such as temporal areas TE3 or perirhinal cortex (PRh), and quantitative analyses were performed on labeling within the subregions of the auditory thalamus in rats. Following LA injections, retrogradely labeled cells were most numerous in the posterior intralaminar nucleus (PIN; 48.0% of all labeled thalamic cells), whereas labeled cells following injections of the entire AAC were most numerous in the dorsal division of the medial geniculate nucleus (MGd; 32.9% of all labeled thalamic cells). Following AAC injections localized to only TE3, the MGd again had the majority of labeled cells (35.9%), whereas following AAC injections localized to PRh, the PIN had the most labeled cells (32.8%). Double-labeled cells were found in all the thalamic regions studied and were most commonly observed in the PIN (43.7% of all double-labeled cells following injections into LA and throughout the AAC). The percentage of double-labeled cells as a proportion of either LA-projecting or AAC-projecting cells varied among the thalamic nuclei studied, ranging from 2.9% up to 42.4%. The topographic distribution of double-labeled cells in the thalamic nuclei resembled that of single-labeled cells following LA injections more than single-labeled cells following AAC injection. These findings suggest that double-labeled cells contribute substantially to many of the direct thalamo-amygdala and indirect thalamo-AAC-amygdala projections. Among other functions, these bifurcating cells may help regulate the processing of input to the LA arriving from these two pathways to allow for certain types of plasticity in the LA during fear conditioning. (C) 2000 Wiley-Liss, Inc.

AB - Auditory information from the posterior thalamus reaches the lateral nucleus of the amygdala (LA) by way of two pathways: A direct thalamo-amygdala projection and a polysynaptic thalamo-cortico-amygdala projection. However, the quantitative extent of thalamic neurons that project to the LA or to the auditory association cortex (AAC) is not known. Furthermore, the extent and topographical distribution of bifurcating cells that project to both LA and AAC are also unknown. Therefore, separate tracers were injected into LA and either into all of AAC or within discrete regions of AAC, such as temporal areas TE3 or perirhinal cortex (PRh), and quantitative analyses were performed on labeling within the subregions of the auditory thalamus in rats. Following LA injections, retrogradely labeled cells were most numerous in the posterior intralaminar nucleus (PIN; 48.0% of all labeled thalamic cells), whereas labeled cells following injections of the entire AAC were most numerous in the dorsal division of the medial geniculate nucleus (MGd; 32.9% of all labeled thalamic cells). Following AAC injections localized to only TE3, the MGd again had the majority of labeled cells (35.9%), whereas following AAC injections localized to PRh, the PIN had the most labeled cells (32.8%). Double-labeled cells were found in all the thalamic regions studied and were most commonly observed in the PIN (43.7% of all double-labeled cells following injections into LA and throughout the AAC). The percentage of double-labeled cells as a proportion of either LA-projecting or AAC-projecting cells varied among the thalamic nuclei studied, ranging from 2.9% up to 42.4%. The topographic distribution of double-labeled cells in the thalamic nuclei resembled that of single-labeled cells following LA injections more than single-labeled cells following AAC injection. These findings suggest that double-labeled cells contribute substantially to many of the direct thalamo-amygdala and indirect thalamo-AAC-amygdala projections. Among other functions, these bifurcating cells may help regulate the processing of input to the LA arriving from these two pathways to allow for certain types of plasticity in the LA during fear conditioning. (C) 2000 Wiley-Liss, Inc.

KW - Auditory cortex

KW - Fear conditioning

KW - Lateral posterior nucleus

KW - Medial geniculate nucleus

KW - Perirhinal cortex

KW - Posterior intralaminar nucleus

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