Cellogram: On-the-Fly Traction Force Microscopy

Tobias Lendenmann, Teseo Schneider, Jérémie Dumas, Marco Tarini, Costanza Giampietro, Apratim Bajpai, Weiqiang Chen, Julia Gerber, Dimos Poulikakos, Aldo Ferrari, Daniele Panozzo

Research output: Contribution to journalArticle

Abstract

Traction force microscopy (TFM) derives maps of cell-generated forces, typically in the nanonewton range, transmitted to the extracellular environment upon actuation of complex biological processes. In traditional approaches, force rendering requires a terminal, time-consuming step of cell deadhesion to obtain a reference image. A conceptually opposite approach is provided by reference-free methods, opening to the on-the-fly generation of force maps from an ongoing experiment. This requires an image processing algorithm keeping the pace of the biological phenomena under investigation. Here, we introduce an integrated software pipeline rendering force maps from single reference-free TFM images seconds to minutes after their acquisition. The algorithm tackles image processing, reference image estimation, and finite element analysis as a single problem, yielding a robust and fully automatic solution. The method's capabilities are demonstrated in two applications. First, the mechanical annihilation of cancer cells is monitored as a function of rising environmental temperature, setting a population threshold at 45 °C. Second, the fast temporal correlation of forces produced across individual cells is used to map physically connected adhesion points, yielding typical lengths that vary as a function of the cell cycle phase.

Original languageEnglish (US)
Pages (from-to)6742-6750
Number of pages9
JournalNano Letters
Volume19
Issue number10
DOIs
StatePublished - Oct 9 2019

Fingerprint

traction
Microscopic examination
microscopy
Image processing
Cells
image processing
cells
Adhesion
Pipelines
Finite element method
actuation
ambient temperature
acquisition
adhesion
cancer
computer programs
Experiments
cycles
thresholds
Temperature

Keywords

  • cTFM
  • focal adhesion
  • Real time analysis
  • reference free
  • stress fibers
  • traction force microscopy

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Lendenmann, T., Schneider, T., Dumas, J., Tarini, M., Giampietro, C., Bajpai, A., ... Panozzo, D. (2019). Cellogram: On-the-Fly Traction Force Microscopy. Nano Letters, 19(10), 6742-6750. https://doi.org/10.1021/acs.nanolett.9b01505

Cellogram : On-the-Fly Traction Force Microscopy. / Lendenmann, Tobias; Schneider, Teseo; Dumas, Jérémie; Tarini, Marco; Giampietro, Costanza; Bajpai, Apratim; Chen, Weiqiang; Gerber, Julia; Poulikakos, Dimos; Ferrari, Aldo; Panozzo, Daniele.

In: Nano Letters, Vol. 19, No. 10, 09.10.2019, p. 6742-6750.

Research output: Contribution to journalArticle

Lendenmann, T, Schneider, T, Dumas, J, Tarini, M, Giampietro, C, Bajpai, A, Chen, W, Gerber, J, Poulikakos, D, Ferrari, A & Panozzo, D 2019, 'Cellogram: On-the-Fly Traction Force Microscopy', Nano Letters, vol. 19, no. 10, pp. 6742-6750. https://doi.org/10.1021/acs.nanolett.9b01505
Lendenmann T, Schneider T, Dumas J, Tarini M, Giampietro C, Bajpai A et al. Cellogram: On-the-Fly Traction Force Microscopy. Nano Letters. 2019 Oct 9;19(10):6742-6750. https://doi.org/10.1021/acs.nanolett.9b01505
Lendenmann, Tobias ; Schneider, Teseo ; Dumas, Jérémie ; Tarini, Marco ; Giampietro, Costanza ; Bajpai, Apratim ; Chen, Weiqiang ; Gerber, Julia ; Poulikakos, Dimos ; Ferrari, Aldo ; Panozzo, Daniele. / Cellogram : On-the-Fly Traction Force Microscopy. In: Nano Letters. 2019 ; Vol. 19, No. 10. pp. 6742-6750.
@article{762dab61d139456c91bff0b24ab894a6,
title = "Cellogram: On-the-Fly Traction Force Microscopy",
abstract = "Traction force microscopy (TFM) derives maps of cell-generated forces, typically in the nanonewton range, transmitted to the extracellular environment upon actuation of complex biological processes. In traditional approaches, force rendering requires a terminal, time-consuming step of cell deadhesion to obtain a reference image. A conceptually opposite approach is provided by reference-free methods, opening to the on-the-fly generation of force maps from an ongoing experiment. This requires an image processing algorithm keeping the pace of the biological phenomena under investigation. Here, we introduce an integrated software pipeline rendering force maps from single reference-free TFM images seconds to minutes after their acquisition. The algorithm tackles image processing, reference image estimation, and finite element analysis as a single problem, yielding a robust and fully automatic solution. The method's capabilities are demonstrated in two applications. First, the mechanical annihilation of cancer cells is monitored as a function of rising environmental temperature, setting a population threshold at 45 °C. Second, the fast temporal correlation of forces produced across individual cells is used to map physically connected adhesion points, yielding typical lengths that vary as a function of the cell cycle phase.",
keywords = "cTFM, focal adhesion, Real time analysis, reference free, stress fibers, traction force microscopy",
author = "Tobias Lendenmann and Teseo Schneider and J{\'e}r{\'e}mie Dumas and Marco Tarini and Costanza Giampietro and Apratim Bajpai and Weiqiang Chen and Julia Gerber and Dimos Poulikakos and Aldo Ferrari and Daniele Panozzo",
year = "2019",
month = "10",
day = "9",
doi = "10.1021/acs.nanolett.9b01505",
language = "English (US)",
volume = "19",
pages = "6742--6750",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "American Chemical Society",
number = "10",

}

TY - JOUR

T1 - Cellogram

T2 - On-the-Fly Traction Force Microscopy

AU - Lendenmann, Tobias

AU - Schneider, Teseo

AU - Dumas, Jérémie

AU - Tarini, Marco

AU - Giampietro, Costanza

AU - Bajpai, Apratim

AU - Chen, Weiqiang

AU - Gerber, Julia

AU - Poulikakos, Dimos

AU - Ferrari, Aldo

AU - Panozzo, Daniele

PY - 2019/10/9

Y1 - 2019/10/9

N2 - Traction force microscopy (TFM) derives maps of cell-generated forces, typically in the nanonewton range, transmitted to the extracellular environment upon actuation of complex biological processes. In traditional approaches, force rendering requires a terminal, time-consuming step of cell deadhesion to obtain a reference image. A conceptually opposite approach is provided by reference-free methods, opening to the on-the-fly generation of force maps from an ongoing experiment. This requires an image processing algorithm keeping the pace of the biological phenomena under investigation. Here, we introduce an integrated software pipeline rendering force maps from single reference-free TFM images seconds to minutes after their acquisition. The algorithm tackles image processing, reference image estimation, and finite element analysis as a single problem, yielding a robust and fully automatic solution. The method's capabilities are demonstrated in two applications. First, the mechanical annihilation of cancer cells is monitored as a function of rising environmental temperature, setting a population threshold at 45 °C. Second, the fast temporal correlation of forces produced across individual cells is used to map physically connected adhesion points, yielding typical lengths that vary as a function of the cell cycle phase.

AB - Traction force microscopy (TFM) derives maps of cell-generated forces, typically in the nanonewton range, transmitted to the extracellular environment upon actuation of complex biological processes. In traditional approaches, force rendering requires a terminal, time-consuming step of cell deadhesion to obtain a reference image. A conceptually opposite approach is provided by reference-free methods, opening to the on-the-fly generation of force maps from an ongoing experiment. This requires an image processing algorithm keeping the pace of the biological phenomena under investigation. Here, we introduce an integrated software pipeline rendering force maps from single reference-free TFM images seconds to minutes after their acquisition. The algorithm tackles image processing, reference image estimation, and finite element analysis as a single problem, yielding a robust and fully automatic solution. The method's capabilities are demonstrated in two applications. First, the mechanical annihilation of cancer cells is monitored as a function of rising environmental temperature, setting a population threshold at 45 °C. Second, the fast temporal correlation of forces produced across individual cells is used to map physically connected adhesion points, yielding typical lengths that vary as a function of the cell cycle phase.

KW - cTFM

KW - focal adhesion

KW - Real time analysis

KW - reference free

KW - stress fibers

KW - traction force microscopy

UR - http://www.scopus.com/inward/record.url?scp=85073067481&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073067481&partnerID=8YFLogxK

U2 - 10.1021/acs.nanolett.9b01505

DO - 10.1021/acs.nanolett.9b01505

M3 - Article

C2 - 31538794

AN - SCOPUS:85073067481

VL - 19

SP - 6742

EP - 6750

JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 10

ER -