Cell membrane translocation of the N-terminal (1-28) part of the prion protein

P. Lundberg, Mazin Magzoub, M. Lindberg, M. Hällbrink, J. Jarvet, L. E.G. Eriksson, Ü Langel, A. Gräslund

Research output: Contribution to journalArticle

Abstract

The N-terminal (1-28) part of the mouse prion protein (PrP) is a cell penetrating peptide, capable of transporting large hydrophilic cargoes through a cell membrane. Confocal fluorescence microscopy shows that it transports the protein avidin (67 kDa) into several cell lines. The (1-28) peptide has a strong tendency for aggregation and β-structure formation, particularly in interaction with negatively charged phospholipid membranes. The findings have implications for how prion proteins with uncleaved signal peptides in the N-termini may enter into cells, which is important for infection. The secondary structure conversion into β-structure may be relevant as a seed for the conversion into the scrapie (PrPSc) form of the protein and its amyloidic transformation.

Original languageEnglish (US)
Pages (from-to)85-90
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume299
Issue number1
DOIs
StatePublished - Dec 2 2002

Fingerprint

PrPSc Proteins
Cell-Penetrating Peptides
Scrapie
Avidin
Confocal microscopy
Fluorescence microscopy
Cell membranes
Protein Sorting Signals
Fluorescence Microscopy
Confocal Microscopy
Seed
Phospholipids
Seeds
Carrier Proteins
Agglomeration
Cells
Cell Membrane
Membranes
Cell Line
Peptides

Keywords

  • β-Structure
  • Aggregation
  • Cell penetrating peptide
  • Membrane interaction
  • Prion protein N-terminus

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cell membrane translocation of the N-terminal (1-28) part of the prion protein. / Lundberg, P.; Magzoub, Mazin; Lindberg, M.; Hällbrink, M.; Jarvet, J.; Eriksson, L. E.G.; Langel, Ü; Gräslund, A.

In: Biochemical and Biophysical Research Communications, Vol. 299, No. 1, 02.12.2002, p. 85-90.

Research output: Contribution to journalArticle

Lundberg, P, Magzoub, M, Lindberg, M, Hällbrink, M, Jarvet, J, Eriksson, LEG, Langel, Ü & Gräslund, A 2002, 'Cell membrane translocation of the N-terminal (1-28) part of the prion protein', Biochemical and Biophysical Research Communications, vol. 299, no. 1, pp. 85-90. https://doi.org/10.1016/S0006-291X(02)02595-0
Lundberg, P. ; Magzoub, Mazin ; Lindberg, M. ; Hällbrink, M. ; Jarvet, J. ; Eriksson, L. E.G. ; Langel, Ü ; Gräslund, A. / Cell membrane translocation of the N-terminal (1-28) part of the prion protein. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 299, No. 1. pp. 85-90.
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