Cell immobilization in gelatin-hydroxyphenylpropionic acid hydrogel fibers

Min Hu, Motoichi Kurisawa, Rensheng Deng, Jeremy Teo, Annegret Schumacher, Ya Xuan Thong, Lishan Wang, Karl M. Schumacher, Jackie Y. Ying

    Research output: Contribution to journalArticle

    Abstract

    Gelatin-hydroxyphenylpropionic acid (Gtn-HPA) hydrogels are highly porous and biodegradable materials. Herein we report a fiber spinning method that can produce cell-seeded solid and hollow hydrogel fibers by enzymatically cross-linking Gtn-HPA in solutions flowing within a capillary tube. The cell-immobilized hydrogel fibers, with feature sizes down to 20 μm, are formed as a result of continuous cross-linking of cell-mixed hydrogel precursors in a multiphase laminar flow. This fiber formation process is mild enough to retain the cell viability. The continuous fiber formation, simultaneous cell encapsulation, as well as versatile combination of fiber structures provided by this approach make it a promising and effective technique for the preparation of cell-seeded hydrogel scaffolds and carriers for tissue engineering.

    Original languageEnglish (US)
    Pages (from-to)3523-3531
    Number of pages9
    JournalBiomaterials
    Volume30
    Issue number21
    DOIs
    StatePublished - Jul 1 2009

    Fingerprint

    Cell immobilization
    Hydrogel
    Gelatin
    Hydrogels
    Immobilization
    Acids
    Fibers
    Immobilized Cells
    Tissue Engineering
    Capillary tubes
    Cell Survival
    Scaffolds (biology)
    Laminar flow
    Encapsulation
    Tissue engineering
    Cells

    Keywords

    • Cell encapsulation
    • Cross-linking
    • Gelatin
    • Hydrogel
    • Scaffold

    ASJC Scopus subject areas

    • Bioengineering
    • Ceramics and Composites
    • Biophysics
    • Biomaterials
    • Mechanics of Materials

    Cite this

    Hu, M., Kurisawa, M., Deng, R., Teo, J., Schumacher, A., Thong, Y. X., ... Ying, J. Y. (2009). Cell immobilization in gelatin-hydroxyphenylpropionic acid hydrogel fibers. Biomaterials, 30(21), 3523-3531. https://doi.org/10.1016/j.biomaterials.2009.03.004

    Cell immobilization in gelatin-hydroxyphenylpropionic acid hydrogel fibers. / Hu, Min; Kurisawa, Motoichi; Deng, Rensheng; Teo, Jeremy; Schumacher, Annegret; Thong, Ya Xuan; Wang, Lishan; Schumacher, Karl M.; Ying, Jackie Y.

    In: Biomaterials, Vol. 30, No. 21, 01.07.2009, p. 3523-3531.

    Research output: Contribution to journalArticle

    Hu, M, Kurisawa, M, Deng, R, Teo, J, Schumacher, A, Thong, YX, Wang, L, Schumacher, KM & Ying, JY 2009, 'Cell immobilization in gelatin-hydroxyphenylpropionic acid hydrogel fibers', Biomaterials, vol. 30, no. 21, pp. 3523-3531. https://doi.org/10.1016/j.biomaterials.2009.03.004
    Hu M, Kurisawa M, Deng R, Teo J, Schumacher A, Thong YX et al. Cell immobilization in gelatin-hydroxyphenylpropionic acid hydrogel fibers. Biomaterials. 2009 Jul 1;30(21):3523-3531. https://doi.org/10.1016/j.biomaterials.2009.03.004
    Hu, Min ; Kurisawa, Motoichi ; Deng, Rensheng ; Teo, Jeremy ; Schumacher, Annegret ; Thong, Ya Xuan ; Wang, Lishan ; Schumacher, Karl M. ; Ying, Jackie Y. / Cell immobilization in gelatin-hydroxyphenylpropionic acid hydrogel fibers. In: Biomaterials. 2009 ; Vol. 30, No. 21. pp. 3523-3531.
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