Cell free translation of chicken calcitonin messenger RNA

F. Lasmoles, C. Desplan, N. Segond, A. Jullienne, F. Treilhou-Lahille, G. Milhaud, M. S. Moukhtar

Research output: Contribution to journalArticle

Abstract

The primary step of calcitonin biosynthesis was studied in a normal organ: chicken ultimobranchial gland, a tissue particularly rich in calcitonin secretory cells. Poly(A)-rich RNA was extracted and purified from ultimobranchial organs and translated in a reticulocyte lysate in the presence of labelled methionine. Polyacrylamide gel electrophoresis of specific immunoprecipitates revealed a major band of Mr 14 500 and a band of Mr 13 300. Thus, in chicken the precursor of calcitonin is a Mr 14500 polypeptide. The minor component of Mr 13 300 could represent limited processing by the reticulocyte lysate.

Original languageEnglish (US)
Pages (from-to)100-104
Number of pages5
JournalFEBS Letters
Volume157
Issue number1
DOIs
StatePublished - Jun 27 1983

Fingerprint

Calcitonin
Chickens
Reticulocytes
Messenger RNA
Poly A
Biosynthesis
Electrophoresis
Methionine
Polyacrylamide Gel Electrophoresis
RNA
Tissue
Peptides
Processing

Keywords

  • Calcitonin
  • Chicken
  • Messenger RNA
  • Radioimmunoassay
  • Translation
  • Ultimobranchial gland

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Lasmoles, F., Desplan, C., Segond, N., Jullienne, A., Treilhou-Lahille, F., Milhaud, G., & Moukhtar, M. S. (1983). Cell free translation of chicken calcitonin messenger RNA. FEBS Letters, 157(1), 100-104. https://doi.org/10.1016/0014-5793(83)81124-7

Cell free translation of chicken calcitonin messenger RNA. / Lasmoles, F.; Desplan, C.; Segond, N.; Jullienne, A.; Treilhou-Lahille, F.; Milhaud, G.; Moukhtar, M. S.

In: FEBS Letters, Vol. 157, No. 1, 27.06.1983, p. 100-104.

Research output: Contribution to journalArticle

Lasmoles, F, Desplan, C, Segond, N, Jullienne, A, Treilhou-Lahille, F, Milhaud, G & Moukhtar, MS 1983, 'Cell free translation of chicken calcitonin messenger RNA', FEBS Letters, vol. 157, no. 1, pp. 100-104. https://doi.org/10.1016/0014-5793(83)81124-7
Lasmoles F, Desplan C, Segond N, Jullienne A, Treilhou-Lahille F, Milhaud G et al. Cell free translation of chicken calcitonin messenger RNA. FEBS Letters. 1983 Jun 27;157(1):100-104. https://doi.org/10.1016/0014-5793(83)81124-7
Lasmoles, F. ; Desplan, C. ; Segond, N. ; Jullienne, A. ; Treilhou-Lahille, F. ; Milhaud, G. ; Moukhtar, M. S. / Cell free translation of chicken calcitonin messenger RNA. In: FEBS Letters. 1983 ; Vol. 157, No. 1. pp. 100-104.
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