Cell cycling and DNA replication in a mutant blocked in cell division in the nematode Caenorhabditis elegans

Donna Albertson, John E. Sulston, John G. White

Research output: Contribution to journalArticle

Abstract

The postembryonic development of the nematode Caenorhabditis elegans has been described at the level of individual cell lineages. A mutant of postembryonic development, lin-5 II, causes a failure of postembryonic nuclear and cell divisions. Mitosis in living animals is seen by light microscopy to proceed through prophase and nuclear envelope breakdown, but an abnormal-looking metaphase plate forms in the mutant, after which the interphase nuclear morphology reappears until the next attempted round of division. The precursor cells which give rise to the ventral nerve cord have been studied in lin-5. In the wild type these cells divide asymmetrically to give six descendants (one hypodermal cell and five neurons). In the mutant these precursors accumulate approximately six times the diploid quantity of DNA within a single nucleus, while attempting mitosis up to three times. These polyploid cells display characteristics of the cells they would have produced ordinarily.

Original languageEnglish (US)
Pages (from-to)165-178
Number of pages14
JournalDevelopmental Biology
Volume63
Issue number1
DOIs
StatePublished - 1978

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Caenorhabditis elegans
DNA Replication
Cell Division
Mitosis
Cell Nucleus Division
Prophase
Polyploidy
Interphase
Nuclear Envelope
Cell Lineage
Metaphase
Diploidy
Microscopy
Neurons
Light
DNA

ASJC Scopus subject areas

  • Developmental Biology

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Cell cycling and DNA replication in a mutant blocked in cell division in the nematode Caenorhabditis elegans. / Albertson, Donna; Sulston, John E.; White, John G.

In: Developmental Biology, Vol. 63, No. 1, 1978, p. 165-178.

Research output: Contribution to journalArticle

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