C/EBP is an immediate-early gene required for the consolidation of long-term facilitation in Aplysia

Cristina Alberini, Mirella Ghirardi, Richard Metz, Eric R. Kandel

Research output: Contribution to journalArticle

Abstract

The consolidation of long-term memory requires protein and mRNA synthesis. A similar requirement has been demonstrated for learning-related synaptic plasticity in the gill-withdrawal reflex of Aplysia. The mono-synaptic component of this reflex can be reconstituted in vitro, where it undergoes both short- and long-term Increases in synaptic strength In response to serotonin (5-HT), a neurotransmitter released during behavioral sensitization, a simple form of learning. As with sensitization, the long-term synaptic modification is characterized by a brief consolidation period during which gene expression Is required. We find that during this phase, the transcription factor Aplysia CCAAT enhancer-binding protein (ApC/EBP) is Induced rapidly by 5-HT and by cAMP, even in the presence of protein synthesis inhibitors. Blocking the function of ApC/EBP blocks long-term facilitation selectively without affecting the short-term process. These data indicate that cAMP-inducible immediate-early genes have an essential role in the consolidation of stable long-term synaptic plasticity in Aplysia.

Original languageEnglish (US)
Pages (from-to)1099-1114
Number of pages16
JournalCell
Volume76
Issue number6
StatePublished - 1994

Fingerprint

Aplysia
Immediate-Early Genes
Consolidation
CCAAT-Enhancer-Binding Proteins
Serotonin
Genes
Neuronal Plasticity
Plasticity
Reflex
Protein Synthesis Inhibitors
Learning
Gene expression
Neurotransmitter Agents
Long-Term Memory
Transcription Factors
Data storage equipment
Messenger RNA
Gene Expression
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

C/EBP is an immediate-early gene required for the consolidation of long-term facilitation in Aplysia. / Alberini, Cristina; Ghirardi, Mirella; Metz, Richard; Kandel, Eric R.

In: Cell, Vol. 76, No. 6, 1994, p. 1099-1114.

Research output: Contribution to journalArticle

Alberini, C, Ghirardi, M, Metz, R & Kandel, ER 1994, 'C/EBP is an immediate-early gene required for the consolidation of long-term facilitation in Aplysia', Cell, vol. 76, no. 6, pp. 1099-1114.
Alberini, Cristina ; Ghirardi, Mirella ; Metz, Richard ; Kandel, Eric R. / C/EBP is an immediate-early gene required for the consolidation of long-term facilitation in Aplysia. In: Cell. 1994 ; Vol. 76, No. 6. pp. 1099-1114.
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