Ccp1 Homodimer Mediates Chromatin Integrity by Antagonizing CENP-A Loading

Qianhua Dong, Feng Xiang Yin, Feng Gao, Yuan Shen, Faben Zhang, Yang Li, Haijin He, Marlyn Gonzalez, Jinpu Yang, Shu Zhang, Min Su, Yu Hang Chen, Fei Li

Research output: Contribution to journalArticle

Abstract

CENP-A is a centromere-specific histone 3 variant essential for centromere specification. CENP-A partially replaces canonical histone H3 at the centromeres. How the particular CENP-A/H3 ratio at centromeres is precisely maintained is unknown. It also remains unclear how CENP-A is excluded from non-centromeric chromatin. Here, we identify Ccp1, an uncharacterized NAP family protein in fission yeast that antagonizes CENP-A loading at both centromeric and non-centromeric regions. Like the CENP-A loading factor HJURP, Ccp1 interacts with CENP-A and is recruited to centromeres at the end of mitosis in a Mis16-dependent manner. These data indicate that factors with opposing CENP-A loading activities are recruited to centromeres. Furthermore, Ccp1 also cooperates with H2A.Z to evict CENP-A assembled in euchromatin. Structural analyses indicate that Ccp1 forms a homodimer that is required for its anti-CENP-A loading activity. Our study establishes mechanisms for maintenance of CENP-A homeostasis at centromeres and the prevention of ectopic assembly of centromeres.

Original languageEnglish (US)
Pages (from-to)79-91
Number of pages13
JournalMolecular Cell
Volume64
Issue number1
DOIs
StatePublished - Oct 6 2016

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Chromatin
Centromere
Histones
centromere protein A
Schizosaccharomyces pombe Proteins
Euchromatin
Mitosis
Homeostasis
Maintenance

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Ccp1 Homodimer Mediates Chromatin Integrity by Antagonizing CENP-A Loading. / Dong, Qianhua; Yin, Feng Xiang; Gao, Feng; Shen, Yuan; Zhang, Faben; Li, Yang; He, Haijin; Gonzalez, Marlyn; Yang, Jinpu; Zhang, Shu; Su, Min; Chen, Yu Hang; Li, Fei.

In: Molecular Cell, Vol. 64, No. 1, 06.10.2016, p. 79-91.

Research output: Contribution to journalArticle

Dong, Q, Yin, FX, Gao, F, Shen, Y, Zhang, F, Li, Y, He, H, Gonzalez, M, Yang, J, Zhang, S, Su, M, Chen, YH & Li, F 2016, 'Ccp1 Homodimer Mediates Chromatin Integrity by Antagonizing CENP-A Loading', Molecular Cell, vol. 64, no. 1, pp. 79-91. https://doi.org/10.1016/j.molcel.2016.08.022
Dong, Qianhua ; Yin, Feng Xiang ; Gao, Feng ; Shen, Yuan ; Zhang, Faben ; Li, Yang ; He, Haijin ; Gonzalez, Marlyn ; Yang, Jinpu ; Zhang, Shu ; Su, Min ; Chen, Yu Hang ; Li, Fei. / Ccp1 Homodimer Mediates Chromatin Integrity by Antagonizing CENP-A Loading. In: Molecular Cell. 2016 ; Vol. 64, No. 1. pp. 79-91.
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