Cathepsin B and L activities in isolated osteoclasts

Barry R. Rifkin, Anthony T. Vernillo, Audrey P. Kleckner, Jeanine M. Auszmann, Linda Rosenberg, Morris Zimmerman

Research output: Contribution to journalArticle

Abstract

Cathepsin B and L activities were examined with chicken osteoclasts isolated by sequential filtration and inhibitors were added to disaggregated rat osteoclasts on cortical bovine bone. Z-Phe-Phe-CHN2, a selective inhibitor of cathepsin L, at 1, 5, and 10 μM, inhibited bone resorption by rat osteoclasts 50, 85, and 100 per cent and, in chicken osteoclasts, cathepsin L activity was comparably inhibited. Cathepsin L in avian osteoclasts was also 25-fold higher than cathepsin B. Chicken osteoclasts treated with Z-Phe-Ala-CHN2, a generalized cysteine proteinase inhibitor, had both cathepsins inhibited to the same extent. Cathepsin L may play a key role in resorption.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume179
Issue number1
DOIs
StatePublished - Aug 30 1991

Fingerprint

Cathepsin L
Cathepsin B
Osteoclasts
Chickens
Rats
Bone
Cysteine Proteinase Inhibitors
Cathepsins
Bone Resorption

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Rifkin, B. R., Vernillo, A. T., Kleckner, A. P., Auszmann, J. M., Rosenberg, L., & Zimmerman, M. (1991). Cathepsin B and L activities in isolated osteoclasts. Biochemical and Biophysical Research Communications, 179(1), 63-69. https://doi.org/10.1016/0006-291X(91)91334-9

Cathepsin B and L activities in isolated osteoclasts. / Rifkin, Barry R.; Vernillo, Anthony T.; Kleckner, Audrey P.; Auszmann, Jeanine M.; Rosenberg, Linda; Zimmerman, Morris.

In: Biochemical and Biophysical Research Communications, Vol. 179, No. 1, 30.08.1991, p. 63-69.

Research output: Contribution to journalArticle

Rifkin, BR, Vernillo, AT, Kleckner, AP, Auszmann, JM, Rosenberg, L & Zimmerman, M 1991, 'Cathepsin B and L activities in isolated osteoclasts', Biochemical and Biophysical Research Communications, vol. 179, no. 1, pp. 63-69. https://doi.org/10.1016/0006-291X(91)91334-9
Rifkin, Barry R. ; Vernillo, Anthony T. ; Kleckner, Audrey P. ; Auszmann, Jeanine M. ; Rosenberg, Linda ; Zimmerman, Morris. / Cathepsin B and L activities in isolated osteoclasts. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 179, No. 1. pp. 63-69.
@article{4891eeb7ed5247a8a20646792fc1654b,
title = "Cathepsin B and L activities in isolated osteoclasts",
abstract = "Cathepsin B and L activities were examined with chicken osteoclasts isolated by sequential filtration and inhibitors were added to disaggregated rat osteoclasts on cortical bovine bone. Z-Phe-Phe-CHN2, a selective inhibitor of cathepsin L, at 1, 5, and 10 μM, inhibited bone resorption by rat osteoclasts 50, 85, and 100 per cent and, in chicken osteoclasts, cathepsin L activity was comparably inhibited. Cathepsin L in avian osteoclasts was also 25-fold higher than cathepsin B. Chicken osteoclasts treated with Z-Phe-Ala-CHN2, a generalized cysteine proteinase inhibitor, had both cathepsins inhibited to the same extent. Cathepsin L may play a key role in resorption.",
author = "Rifkin, {Barry R.} and Vernillo, {Anthony T.} and Kleckner, {Audrey P.} and Auszmann, {Jeanine M.} and Linda Rosenberg and Morris Zimmerman",
year = "1991",
month = "8",
day = "30",
doi = "10.1016/0006-291X(91)91334-9",
language = "English (US)",
volume = "179",
pages = "63--69",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Cathepsin B and L activities in isolated osteoclasts

AU - Rifkin, Barry R.

AU - Vernillo, Anthony T.

AU - Kleckner, Audrey P.

AU - Auszmann, Jeanine M.

AU - Rosenberg, Linda

AU - Zimmerman, Morris

PY - 1991/8/30

Y1 - 1991/8/30

N2 - Cathepsin B and L activities were examined with chicken osteoclasts isolated by sequential filtration and inhibitors were added to disaggregated rat osteoclasts on cortical bovine bone. Z-Phe-Phe-CHN2, a selective inhibitor of cathepsin L, at 1, 5, and 10 μM, inhibited bone resorption by rat osteoclasts 50, 85, and 100 per cent and, in chicken osteoclasts, cathepsin L activity was comparably inhibited. Cathepsin L in avian osteoclasts was also 25-fold higher than cathepsin B. Chicken osteoclasts treated with Z-Phe-Ala-CHN2, a generalized cysteine proteinase inhibitor, had both cathepsins inhibited to the same extent. Cathepsin L may play a key role in resorption.

AB - Cathepsin B and L activities were examined with chicken osteoclasts isolated by sequential filtration and inhibitors were added to disaggregated rat osteoclasts on cortical bovine bone. Z-Phe-Phe-CHN2, a selective inhibitor of cathepsin L, at 1, 5, and 10 μM, inhibited bone resorption by rat osteoclasts 50, 85, and 100 per cent and, in chicken osteoclasts, cathepsin L activity was comparably inhibited. Cathepsin L in avian osteoclasts was also 25-fold higher than cathepsin B. Chicken osteoclasts treated with Z-Phe-Ala-CHN2, a generalized cysteine proteinase inhibitor, had both cathepsins inhibited to the same extent. Cathepsin L may play a key role in resorption.

UR - http://www.scopus.com/inward/record.url?scp=0026055964&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026055964&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(91)91334-9

DO - 10.1016/0006-291X(91)91334-9

M3 - Article

C2 - 1883385

AN - SCOPUS:0026055964

VL - 179

SP - 63

EP - 69

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -