Ca2+ binding properties of type X collagen

Thorsten Kirsch, Klaus von der Mark

    Research output: Contribution to journalArticle

    Abstract

    Type X collagen is a developmentally regulated collagen that is only synthesized by chondrocytes of the hypertrophic and calcifying zone in fetal cartilage. There is evidence in the literature that type X collagen may be involved in cartilage calcification. Here we show that type X collagen synthesis precedes calcium deposition in nodules of fetal human chondrocytes forming in cell culture and present evidence that type X collagen binds calcium in a specific and dose dependent manner. In an assay using bovine type X collagen coupled to beads and 45Ca2+ we determined a total of about 15 binding sites per α1(X) chain with a dissociation constant of 32 μM.

    Original languageEnglish (US)
    Pages (from-to)149-152
    Number of pages4
    JournalFEBS Letters
    Volume294
    Issue number1-2
    DOIs
    StatePublished - Dec 2 1991

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    Keywords

    • Calcium binding
    • Cartilage calcification
    • Chondrocyte hypertrophy
    • Type X collagen

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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