Carcinogen-DNA adducts and gene mutation in foundry workers with low-level exposure to polycyclic aromatic hydrocarbons

R. R. Perera, Chris Dickey, R. Santella, J. P. O'neill, R. J. Albertini, R. Ottman, W. Y. Tsai, L. A. Mooney, K. Savela, K. Hemminki

Research output: Contribution to journalArticle

Abstract

Carcinogen-DNA adducts and somatic gene mutation at the hypoxanthine guanine phosphoribosyl transferase (HPRT) locus were evaluated in peripheral leukocytes of workers in an iron foundry with exposure to benzo[a]pyrene (B[a]P) and other polycyclic aromatic hydrocarbons (PAHs). During the two year study period, B[α]P exposure declined by ∼40%, from a maximum of 60 ng/m3 in the first year to 3 1 year later. A total of 64 persons were sampled in November/December of the two successive study years; 24 of them gave two samples one year apart. The biomarkers included carcinogen-DNA adducts in leukocytes (PAH-DNA measured by an immunoassay, aromatic-DNA by the 32P-postlabeling method) and HPRT mutation in lymphocytes. After adjusting for smoking, levels of PAH-DNA, aromatic-DNA and HPRT mutation frequency (Mf) increased with exposure among the 64 workers sampled during the 2 year period (P ≤ 0.05). However, the markers showed a differential response to the change in exposure, consistent with their individual biology. For example, among the 24 workers sampled in both years, carcinogen-DNA adducts (which have a halflife on the order of several months) were markedly reduced from the first to the second year (PAH-DNA, 6.2 versus 2.3/108; aromatic-DNA, 2.5 versus 1.4/108; P P

Original languageEnglish (US)
Pages (from-to)2905-2910
Number of pages6
JournalCarcinogenesis
Volume15
Issue number12
DOIs
StatePublished - Dec 1994

Fingerprint

Carcinogens
DNA Adducts
Polycyclic Aromatic Hydrocarbons
Foundries
Hydrocarbons
Polycyclic aromatic hydrocarbons
Mutation
DNA
Genes
Gene
Leukocytes
Hypoxanthine
Guanine
Transferases
Immunoassay
Lymphocytes
Smoking
Biomarkers
Iron
Biology

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience
  • Cancer Research

Cite this

Carcinogen-DNA adducts and gene mutation in foundry workers with low-level exposure to polycyclic aromatic hydrocarbons. / Perera, R. R.; Dickey, Chris; Santella, R.; O'neill, J. P.; Albertini, R. J.; Ottman, R.; Tsai, W. Y.; Mooney, L. A.; Savela, K.; Hemminki, K.

In: Carcinogenesis, Vol. 15, No. 12, 12.1994, p. 2905-2910.

Research output: Contribution to journalArticle

Perera, RR, Dickey, C, Santella, R, O'neill, JP, Albertini, RJ, Ottman, R, Tsai, WY, Mooney, LA, Savela, K & Hemminki, K 1994, 'Carcinogen-DNA adducts and gene mutation in foundry workers with low-level exposure to polycyclic aromatic hydrocarbons', Carcinogenesis, vol. 15, no. 12, pp. 2905-2910. https://doi.org/10.1093/carcin/15.12.2905
Perera, R. R. ; Dickey, Chris ; Santella, R. ; O'neill, J. P. ; Albertini, R. J. ; Ottman, R. ; Tsai, W. Y. ; Mooney, L. A. ; Savela, K. ; Hemminki, K. / Carcinogen-DNA adducts and gene mutation in foundry workers with low-level exposure to polycyclic aromatic hydrocarbons. In: Carcinogenesis. 1994 ; Vol. 15, No. 12. pp. 2905-2910.
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