Capillary-valve-based fabrication of ion-selective membrane junction for electrokinetic sample preconcentration in PDMS chip

Vincent Liu, Rafael Song, Jongyoon Han

    Research output: Contribution to journalArticle

    Abstract

    In this paper, we report a novel method for fabricating ion-selective membranes in poly(dimethylsiloxane) (PDMS)/glass-based microfluidic preconcentrators. Based on the concept of capillary valves, this fabrication method involves filling a lithographically patterned junction between two microchannels with an ion-selective material such as Nafion resin; subsequent curing results in a high aspect-ratio membrane for use in electrokinetic sample preconcentration. To demonstrate the concentration performance of this high-aspect-ratio, ion-selective membrane, we integrated the preconcentrator with a surface-based immunoassay for R-Phycoerythrin (RPE). Using a 1× PBS buffer system, the preconcentrator-enhanced immunoassay showed an approximately 100× improvement in sensitivity within 30 min. This is the first time that an electrokinetic microfluidic preconcentrator based on ion concentration polarization (ICP) has been used in high ionic strength buffer solutions to enhance the sensitivity of a surface-based immunoassay.

    Original languageEnglish (US)
    Pages (from-to)1485-1490
    Number of pages6
    JournalLab on a Chip
    Volume10
    Issue number11
    DOIs
    StatePublished - Jan 1 2010

    Fingerprint

    Ion selective membranes
    Polydimethylsiloxane
    Microfluidics
    Aspect ratio
    Buffers
    Immunoassay
    Phycoerythrin
    Ions
    Fabrication
    Membranes
    Microchannels
    Ionic strength
    Curing
    Resins
    Polarization
    Glass
    Osmolar Concentration
    baysilon

    ASJC Scopus subject areas

    • Bioengineering
    • Chemistry(all)
    • Biochemistry
    • Biomedical Engineering

    Cite this

    Capillary-valve-based fabrication of ion-selective membrane junction for electrokinetic sample preconcentration in PDMS chip. / Liu, Vincent; Song, Rafael; Han, Jongyoon.

    In: Lab on a Chip, Vol. 10, No. 11, 01.01.2010, p. 1485-1490.

    Research output: Contribution to journalArticle

    Liu, Vincent ; Song, Rafael ; Han, Jongyoon. / Capillary-valve-based fabrication of ion-selective membrane junction for electrokinetic sample preconcentration in PDMS chip. In: Lab on a Chip. 2010 ; Vol. 10, No. 11. pp. 1485-1490.
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