Calcium modulation of mitochondrial inner membrane channel activity

Kathleen W. Kinnally, Zorov Dmitry, Yuri Antonenko, Sean Perini

Research output: Contribution to journalArticle

Abstract

Protocols were defined that enable patch-clamp studies of the ∼107 pS voltage dependent channel and a class of activity we refer to as MCC (multiconductance channel) which is characterized by multiple levels and transitions as high as 1 to 1.5 nS. If free calcium was kept at 10-7 M or lower during mitochondrial isolation, no activity was observed at low voltage (±60 mV). If free calcium levels were higher, MCC activity was observed in about 96% of the patches. The observation of ∼ 107 pS channel was enhanced from 2% to 68% of patches by washing isolated mitoplasts (mitochondria stripped of outer membrane) with EGTA. Increasing matrix calcium from 10-9 to 10-5 M decreased the probability of opening for the MCC and ∼107 pS activities.

Original languageEnglish (US)
Pages (from-to)1183-1188
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume176
Issue number3
DOIs
StatePublished - May 15 1991

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Ion Channels
Modulation
Calcium
Mitochondria
Egtazic Acid
Clamping devices
Electric potential
Washing
Observation
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Calcium modulation of mitochondrial inner membrane channel activity. / Kinnally, Kathleen W.; Dmitry, Zorov; Antonenko, Yuri; Perini, Sean.

In: Biochemical and Biophysical Research Communications, Vol. 176, No. 3, 15.05.1991, p. 1183-1188.

Research output: Contribution to journalArticle

Kinnally, Kathleen W. ; Dmitry, Zorov ; Antonenko, Yuri ; Perini, Sean. / Calcium modulation of mitochondrial inner membrane channel activity. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 176, No. 3. pp. 1183-1188.
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