C. elegans chromosomes connect to centrosomes by anchoring into the spindle network

Stefanie Redemann, Johannes Baumgart, Norbert Lindow, Michael Shelley, Ehssan Nazockdast, Andrea Kratz, Steffen Prohaska, Jan Brugués, Sebastian Fürthauer, Thomas Müller-Reichert

Research output: Contribution to journalArticle

Abstract

The mitotic spindle ensures the faithful segregation of chromosomes. Here we combine the first large-scale serial electron tomography of whole mitotic spindles in early C. elegans embryos with live-cell imaging to reconstruct all microtubules in 3D and identify their plus- and minus-ends. We classify them as kinetochore (KMTs), spindle (SMTs) or astral microtubules (AMTs) according to their positions, and quantify distinct properties of each class. While our light microscopy and mutant studies show that microtubules are nucleated from the centrosomes, we find only a few KMTs directly connected to the centrosomes. Indeed, by quantitatively analysing several models of microtubule growth, we conclude that minus-ends of KMTs have selectively detached and depolymerized from the centrosome. In toto, our results show that the connection between centrosomes and chromosomes is mediated by an anchoring into the entire spindle network and that any direct connections through KMTs are few and likely very transient.

Original languageEnglish (US)
Article number15288
JournalNature Communications
Volume8
DOIs
StatePublished - Jan 1 2017

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Chromosomes, Human, 6-12 and X
Centrosome
spindles
chromosomes
Chromosomes
Microtubules
Spindle Apparatus
Surface mount technology
Optical microscopy
Tomography
Electron Microscope Tomography
Imaging techniques
Kinetochores
Chromosome Segregation
Electrons
embryos
Microscopy
Embryonic Structures
tomography
microscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Redemann, S., Baumgart, J., Lindow, N., Shelley, M., Nazockdast, E., Kratz, A., ... Müller-Reichert, T. (2017). C. elegans chromosomes connect to centrosomes by anchoring into the spindle network. Nature Communications, 8, [15288]. https://doi.org/10.1038/ncomms15288

C. elegans chromosomes connect to centrosomes by anchoring into the spindle network. / Redemann, Stefanie; Baumgart, Johannes; Lindow, Norbert; Shelley, Michael; Nazockdast, Ehssan; Kratz, Andrea; Prohaska, Steffen; Brugués, Jan; Fürthauer, Sebastian; Müller-Reichert, Thomas.

In: Nature Communications, Vol. 8, 15288, 01.01.2017.

Research output: Contribution to journalArticle

Redemann, S, Baumgart, J, Lindow, N, Shelley, M, Nazockdast, E, Kratz, A, Prohaska, S, Brugués, J, Fürthauer, S & Müller-Reichert, T 2017, 'C. elegans chromosomes connect to centrosomes by anchoring into the spindle network', Nature Communications, vol. 8, 15288. https://doi.org/10.1038/ncomms15288
Redemann S, Baumgart J, Lindow N, Shelley M, Nazockdast E, Kratz A et al. C. elegans chromosomes connect to centrosomes by anchoring into the spindle network. Nature Communications. 2017 Jan 1;8. 15288. https://doi.org/10.1038/ncomms15288
Redemann, Stefanie ; Baumgart, Johannes ; Lindow, Norbert ; Shelley, Michael ; Nazockdast, Ehssan ; Kratz, Andrea ; Prohaska, Steffen ; Brugués, Jan ; Fürthauer, Sebastian ; Müller-Reichert, Thomas. / C. elegans chromosomes connect to centrosomes by anchoring into the spindle network. In: Nature Communications. 2017 ; Vol. 8.
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