Bright, red emitting fluorescent sensor for intracellular imaging of Mg2+

Qitian Lin, Jessica J. Gruskos, Daniela Buccella

Research output: Contribution to journalArticle

Abstract

Fluorescent sensors with low-energy excitation are in great demand for the study of cellular Mg2+ by fluorescence miscroscopy, but to date they remain scarce. Addressing this gap, we report herein a new set of molecular fluorescent sensors for the detection of Mg2+ based on an o-aminophenol-N,N,O-triacetic acid (APTRA) metal-recognition moiety combined with two different BODIPY fluorophores. The new sensors, MagB1 and MagB2, display absorption and emission maxima in the visible range and respond to Mg2+ in aqueous buffer with large fluorescence enhancements. MagB2, a red-emitting fluorescent indicator based on a styryl-BODIPY, displays superior metal selectivity and optical properties compared to its green emitting counterpart, MagB1. With an excellent 58-fold fluorescence turn-on and Mg2+ dissociation constant in tune with physiological concentrations of the cation (low millimolar range), MagB2 enables visualization of changes in intracellular levels of free Mg2+ in live cells with no significant interference from basal levels of Ca2+, the most common competitor.

Original languageEnglish (US)
Pages (from-to)11381-11388
Number of pages8
JournalOrganic and Biomolecular Chemistry
Volume14
Issue number48
DOIs
StatePublished - 2016

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Fluorescence
Imaging techniques
fluorescence
sensors
Sensors
Metals
Fluorophores
Excitation energy
metals
Cations
Buffers
Visualization
Optical properties
buffers
selectivity
Display devices
dissociation
interference
optical properties
cations

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Bright, red emitting fluorescent sensor for intracellular imaging of Mg2+. / Lin, Qitian; Gruskos, Jessica J.; Buccella, Daniela.

In: Organic and Biomolecular Chemistry, Vol. 14, No. 48, 2016, p. 11381-11388.

Research output: Contribution to journalArticle

Lin, Qitian ; Gruskos, Jessica J. ; Buccella, Daniela. / Bright, red emitting fluorescent sensor for intracellular imaging of Mg2+. In: Organic and Biomolecular Chemistry. 2016 ; Vol. 14, No. 48. pp. 11381-11388.
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