Bright, red emitting fluorescent sensor for intracellular imaging of Mg2+

Qitian Lin, Jessica J. Gruskos, Daniela Buccella

Research output: Contribution to journalArticle

Abstract

Fluorescent sensors with low-energy excitation are in great demand for the study of cellular Mg2+ by fluorescence miscroscopy, but to date they remain scarce. Addressing this gap, we report herein a new set of molecular fluorescent sensors for the detection of Mg2+ based on an o-aminophenol-N,N,O-triacetic acid (APTRA) metal-recognition moiety combined with two different BODIPY fluorophores. The new sensors, MagB1 and MagB2, display absorption and emission maxima in the visible range and respond to Mg2+ in aqueous buffer with large fluorescence enhancements. MagB2, a red-emitting fluorescent indicator based on a styryl-BODIPY, displays superior metal selectivity and optical properties compared to its green emitting counterpart, MagB1. With an excellent 58-fold fluorescence turn-on and Mg2+ dissociation constant in tune with physiological concentrations of the cation (low millimolar range), MagB2 enables visualization of changes in intracellular levels of free Mg2+ in live cells with no significant interference from basal levels of Ca2+, the most common competitor.

Original languageEnglish (US)
Pages (from-to)11381-11388
Number of pages8
JournalOrganic and Biomolecular Chemistry
Volume14
Issue number48
DOIs
StatePublished - Jan 1 2016

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this