Breaking the degeneracy of the genetic code

Inchan Kwon, Kent Kirshenbaum, David A. Tirrell

Research output: Contribution to journalArticle

Abstract

A mutant yeast phenylalanine transfer RNA (ytRNAPheAAA) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNAPheAAA and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.

Original languageEnglish (US)
Pages (from-to)7512-7513
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number25
DOIs
StatePublished - Jun 25 2003

Fingerprint

Genetic Code
Phenylalanine
Yeast
Escherichia coli
Phenylalanine-tRNA Ligase
Yeasts
Anticodon
Transfer RNA
Codon
3-(2-naphthyl)alanine

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Breaking the degeneracy of the genetic code. / Kwon, Inchan; Kirshenbaum, Kent; Tirrell, David A.

In: Journal of the American Chemical Society, Vol. 125, No. 25, 25.06.2003, p. 7512-7513.

Research output: Contribution to journalArticle

Kwon, Inchan ; Kirshenbaum, Kent ; Tirrell, David A. / Breaking the degeneracy of the genetic code. In: Journal of the American Chemical Society. 2003 ; Vol. 125, No. 25. pp. 7512-7513.
@article{faf6a6a6200d4043973361b0b4f58f6c,
title = "Breaking the degeneracy of the genetic code",
abstract = "A mutant yeast phenylalanine transfer RNA (ytRNAPheAAA) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNAPheAAA and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.",
author = "Inchan Kwon and Kent Kirshenbaum and Tirrell, {David A.}",
year = "2003",
month = "6",
day = "25",
doi = "10.1021/ja0350076",
language = "English (US)",
volume = "125",
pages = "7512--7513",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "25",

}

TY - JOUR

T1 - Breaking the degeneracy of the genetic code

AU - Kwon, Inchan

AU - Kirshenbaum, Kent

AU - Tirrell, David A.

PY - 2003/6/25

Y1 - 2003/6/25

N2 - A mutant yeast phenylalanine transfer RNA (ytRNAPheAAA) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNAPheAAA and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.

AB - A mutant yeast phenylalanine transfer RNA (ytRNAPheAAA) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNAPheAAA and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.

UR - http://www.scopus.com/inward/record.url?scp=0037870583&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037870583&partnerID=8YFLogxK

U2 - 10.1021/ja0350076

DO - 10.1021/ja0350076

M3 - Article

VL - 125

SP - 7512

EP - 7513

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 25

ER -