Brachyury downstream notochord differentiation in the ascidian embryo

Hiroki Takahashi, Kohji Hotta, Albert Erives, Anna Di Gregorio, Robert W. Zeller, Michael Levine, Nori Satoh

Research output: Contribution to journalArticle

Abstract

The ascidian tadpole represents the most simplified chordate body plan. It contains a notochord composed of just 40 cells, but as in vertebrates Brachyury is essential for notochord differentiation. Here, we show that the misexpression of the Brachyury gene (Ci-Bra) of Ciona intestinalis is sufficient to transform endoderm into notochord. Subtractive hybridization screens were conducted to identify potential Brachyury target genes that are induced upon Ci-Bra misexpression. Of 501 independent cDNA clones that were surveyed, 38 were specifically expressed in notochord cells. These potential Ci-Bra downstream genes appear to encode a broad spectrum of divergent proteins associated with notochord formation.

Original languageEnglish (US)
Pages (from-to)1519-1523
Number of pages5
JournalGenes & development
Volume13
Issue number12
StatePublished - Jun 15 1999

Fingerprint

Notochord
Urochordata
Embryonic Structures
Ciona intestinalis
Chordata
Genes
Endoderm
Larva
Vertebrates
Complementary DNA
Clone Cells
Brachyury protein
Proteins

Keywords

  • Ascidians
  • Brachyury
  • Differentiation
  • Downstream genes
  • Notochord

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Takahashi, H., Hotta, K., Erives, A., Di Gregorio, A., Zeller, R. W., Levine, M., & Satoh, N. (1999). Brachyury downstream notochord differentiation in the ascidian embryo. Genes & development, 13(12), 1519-1523.

Brachyury downstream notochord differentiation in the ascidian embryo. / Takahashi, Hiroki; Hotta, Kohji; Erives, Albert; Di Gregorio, Anna; Zeller, Robert W.; Levine, Michael; Satoh, Nori.

In: Genes & development, Vol. 13, No. 12, 15.06.1999, p. 1519-1523.

Research output: Contribution to journalArticle

Takahashi, H, Hotta, K, Erives, A, Di Gregorio, A, Zeller, RW, Levine, M & Satoh, N 1999, 'Brachyury downstream notochord differentiation in the ascidian embryo', Genes & development, vol. 13, no. 12, pp. 1519-1523.
Takahashi H, Hotta K, Erives A, Di Gregorio A, Zeller RW, Levine M et al. Brachyury downstream notochord differentiation in the ascidian embryo. Genes & development. 1999 Jun 15;13(12):1519-1523.
Takahashi, Hiroki ; Hotta, Kohji ; Erives, Albert ; Di Gregorio, Anna ; Zeller, Robert W. ; Levine, Michael ; Satoh, Nori. / Brachyury downstream notochord differentiation in the ascidian embryo. In: Genes & development. 1999 ; Vol. 13, No. 12. pp. 1519-1523.
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