Borate binding to siderophores: Structure and stability

Wesley R. Harris, Shady Amin, Frithjof C. Küpper, David H. Green, Carl J. Carrano

Research output: Contribution to journalArticle

Abstract

Well-known as specific iron chelating agents produced by bacteria, it is shown that some, but not all, siderophore classes have an unexpected binding affinity for boron. The relevant criterium is the availability of a vicinal dianionic oxygen containing binding group (i.e., citrate or catecholate). The resulting boron complexes have been characterized by ESI-MS, multinuclear NMR, and DFT calculations. Detailed boron binding constants have been measured for vibrioferrin, rhizoferrin, and petrobactin. The observed affinity of certain siderophores for borate, a common chemical species in the marine but not the terrestrial environment, allows for small, but potentially significant, concentrations of B-siderophores to exist at oceanic pH. We hypothesize that these concentrations could be sufficient for them to function as cell signaling molecules or as mediators of biological boron uptake. In addition, binding of the tetrahedral boron to these siderophores results in a conformation that is different from either the free siderophore or its iron complex and would thus allow a distinction to be made between its iron uptake and any putative cell signaling roles.

Original languageEnglish (US)
Pages (from-to)12263-12271
Number of pages9
JournalJournal of the American Chemical Society
Volume129
Issue number40
DOIs
StatePublished - Oct 10 2007

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Siderophores
Borates
Boron
Cell signaling
Iron
Iron Chelating Agents
Biomolecular Nuclear Magnetic Resonance
Chelation
Discrete Fourier transforms
Citric Acid
Conformations
Bacteria
Nuclear magnetic resonance
Availability
Oxygen
Molecules

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Harris, W. R., Amin, S., Küpper, F. C., Green, D. H., & Carrano, C. J. (2007). Borate binding to siderophores: Structure and stability. Journal of the American Chemical Society, 129(40), 12263-12271. https://doi.org/10.1021/ja073788v

Borate binding to siderophores : Structure and stability. / Harris, Wesley R.; Amin, Shady; Küpper, Frithjof C.; Green, David H.; Carrano, Carl J.

In: Journal of the American Chemical Society, Vol. 129, No. 40, 10.10.2007, p. 12263-12271.

Research output: Contribution to journalArticle

Harris, WR, Amin, S, Küpper, FC, Green, DH & Carrano, CJ 2007, 'Borate binding to siderophores: Structure and stability', Journal of the American Chemical Society, vol. 129, no. 40, pp. 12263-12271. https://doi.org/10.1021/ja073788v
Harris, Wesley R. ; Amin, Shady ; Küpper, Frithjof C. ; Green, David H. ; Carrano, Carl J. / Borate binding to siderophores : Structure and stability. In: Journal of the American Chemical Society. 2007 ; Vol. 129, No. 40. pp. 12263-12271.
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