Bone apposition to laminin-1 coated implants: Histologic and 3D evaluation

K. Bougas, R. Jimbo, S. Vandeweghe, M. Hayashi, M. Bryington, Y. Kozai, H. O. Schwartz-Filho, N. Tovar, E. Adolfsson, D. Ono, Paulo Coelho, A. Wennerberg

Research output: Contribution to journalArticle

Abstract

Laminin-1 has been reported as one of the factors responsible for the nucleation of calcium phosphates and, in vitro, has been reported to selectively recruit osteoprogenitors. This article focused on its in vivo effects, and evaluated the effect of laminin-1 local application on osseointegration. Polished cylindrical hydroxyapatite implants were coated with laminin-1 (test) and the bone responses in the rabbit tibiae after 2 and 4 weeks were evaluated and compared to the non-coated implants (control). Before the samples were processed for histological sectioning, they were three-dimensionally analysed with micro computed tomography (μCT). Both evaluation methods were analysed with regards to bone area around the implant and bone to implant contact. From the histologic observation, new bone formation around the laminin-1 coated implant at 2 weeks seemed to have increased the amount of supporting bone around the implant, however, at 4 weeks, the two groups presented no notable differences. The two-dimensional and three-dimensional morphometric evaluation revealed that both histologic and three-dimensional analysis showed some tendency in favour of the test group implants, however there was no statistical significance between the test and control group results.

Original languageEnglish (US)
Pages (from-to)677-682
Number of pages6
JournalInternational Journal of Oral and Maxillofacial Surgery
Volume42
Issue number5
DOIs
StatePublished - May 2013

Fingerprint

Bone and Bones
Calcium Phosphates
Osseointegration
Durapatite
Tibia
Osteogenesis
Tomography
Observation
Rabbits
Control Groups
laminin 1
In Vitro Techniques

Keywords

  • animal experiments
  • bone implant interactions
  • laminin
  • micro CT

ASJC Scopus subject areas

  • Oral Surgery
  • Otorhinolaryngology
  • Surgery

Cite this

Bougas, K., Jimbo, R., Vandeweghe, S., Hayashi, M., Bryington, M., Kozai, Y., ... Wennerberg, A. (2013). Bone apposition to laminin-1 coated implants: Histologic and 3D evaluation. International Journal of Oral and Maxillofacial Surgery, 42(5), 677-682. https://doi.org/10.1016/j.ijom.2012.11.008

Bone apposition to laminin-1 coated implants : Histologic and 3D evaluation. / Bougas, K.; Jimbo, R.; Vandeweghe, S.; Hayashi, M.; Bryington, M.; Kozai, Y.; Schwartz-Filho, H. O.; Tovar, N.; Adolfsson, E.; Ono, D.; Coelho, Paulo; Wennerberg, A.

In: International Journal of Oral and Maxillofacial Surgery, Vol. 42, No. 5, 05.2013, p. 677-682.

Research output: Contribution to journalArticle

Bougas, K, Jimbo, R, Vandeweghe, S, Hayashi, M, Bryington, M, Kozai, Y, Schwartz-Filho, HO, Tovar, N, Adolfsson, E, Ono, D, Coelho, P & Wennerberg, A 2013, 'Bone apposition to laminin-1 coated implants: Histologic and 3D evaluation', International Journal of Oral and Maxillofacial Surgery, vol. 42, no. 5, pp. 677-682. https://doi.org/10.1016/j.ijom.2012.11.008
Bougas, K. ; Jimbo, R. ; Vandeweghe, S. ; Hayashi, M. ; Bryington, M. ; Kozai, Y. ; Schwartz-Filho, H. O. ; Tovar, N. ; Adolfsson, E. ; Ono, D. ; Coelho, Paulo ; Wennerberg, A. / Bone apposition to laminin-1 coated implants : Histologic and 3D evaluation. In: International Journal of Oral and Maxillofacial Surgery. 2013 ; Vol. 42, No. 5. pp. 677-682.
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