Block liposomes from curvature-stabilizing lipids: Connected nanotubes, -rods, or -spheres

Alexandra Zidovska, Kai K. Ewert, Joel Quispe, Bridget Carragher, Clinton S. Potter, Cyrus R. Safinya

    Research output: Contribution to journalArticle

    Abstract

    We report on the discovery of block liposomes, a new class of chain-melted (liquid) vesicles, with membranes comprised of mixtures of the membrane-curvature-stabilizing multivalent lipid MVLBG2 of colossal charge +16 e and neutral l,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC). In a narrow MVLBG2 composition range (8 - 10 mol %), cryo-TEM revealed block liposomes consisting of distinctly shaped, yet connected, nanoscale spheres, pears, tubes, or rods. Unlike typical liposome systems, where spherical vesicles, tubular vesicles, and cylindrical micelles are separated on the macroscopic scale, within a block liposome, shapes are separated on the nanometer scale. Diblock (pear - tube) and triblock (pear - tube - pear) liposomes contain nanotubes with inner lumen diameter of 10 - 50 nm. Diblock (sphere - rod) liposomes were found to contain micellar nanorods ≈4 nm in diameter and several micrometers in length, analogous to cytoskeletal filaments of eukaryotic cells. Block liposomes may find a range of applications in chemical and nucleic acid delivery and as building blocks in the design of templates for hierarchical structures.

    Original languageEnglish (US)
    Pages (from-to)2979-2985
    Number of pages7
    JournalLangmuir
    Volume25
    Issue number5
    DOIs
    StatePublished - Mar 3 2009

    Fingerprint

    Liposomes
    Nanotubes
    Lipids
    lipids
    nanotubes
    rods
    curvature
    tubes
    membranes
    lumens
    nucleic acids
    nanorods
    micrometers
    delivery
    micelles
    filaments
    templates
    transmission electron microscopy
    acids
    Membranes

    ASJC Scopus subject areas

    • Electrochemistry
    • Condensed Matter Physics
    • Surfaces and Interfaces
    • Materials Science(all)
    • Spectroscopy

    Cite this

    Zidovska, A., Ewert, K. K., Quispe, J., Carragher, B., Potter, C. S., & Safinya, C. R. (2009). Block liposomes from curvature-stabilizing lipids: Connected nanotubes, -rods, or -spheres. Langmuir, 25(5), 2979-2985. https://doi.org/10.1021/la8022375

    Block liposomes from curvature-stabilizing lipids : Connected nanotubes, -rods, or -spheres. / Zidovska, Alexandra; Ewert, Kai K.; Quispe, Joel; Carragher, Bridget; Potter, Clinton S.; Safinya, Cyrus R.

    In: Langmuir, Vol. 25, No. 5, 03.03.2009, p. 2979-2985.

    Research output: Contribution to journalArticle

    Zidovska, A, Ewert, KK, Quispe, J, Carragher, B, Potter, CS & Safinya, CR 2009, 'Block liposomes from curvature-stabilizing lipids: Connected nanotubes, -rods, or -spheres', Langmuir, vol. 25, no. 5, pp. 2979-2985. https://doi.org/10.1021/la8022375
    Zidovska A, Ewert KK, Quispe J, Carragher B, Potter CS, Safinya CR. Block liposomes from curvature-stabilizing lipids: Connected nanotubes, -rods, or -spheres. Langmuir. 2009 Mar 3;25(5):2979-2985. https://doi.org/10.1021/la8022375
    Zidovska, Alexandra ; Ewert, Kai K. ; Quispe, Joel ; Carragher, Bridget ; Potter, Clinton S. ; Safinya, Cyrus R. / Block liposomes from curvature-stabilizing lipids : Connected nanotubes, -rods, or -spheres. In: Langmuir. 2009 ; Vol. 25, No. 5. pp. 2979-2985.
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