Biphasic calcium phosphate: A scaffold for growth plate chondrocyte maturation

Cristina Teixeira, Yelena Nemelivsky, Claudia Karkia, Racquel Z. Legeros

Research output: Contribution to journalArticle

Abstract

While skeletal development can occur by either intramembranous or endochondral bone formation, all current tissue engineering approaches for bone repair and regeneration try to mimic intramembranous ossification. In this study, we propose to create an in vitro cartilage template as the transient model for in vivo endochondral bone formation. The goals of this study are to (1) establish a method of growing chondrocytes in a well-characterized macroporous biphasic calcium phosphate (MBCP®) scaffold and (2) induce maturation of chondrocytes grown in the MBCP scaffold. Chondrocytes isolated from chick embryonic tibia were grown on MBCP particles and treated with retinoic acid to induce chondrocyte maturation and extracellular matrix deposition. Chondrocytes were observed to attach and proliferate on the MBCP scaffold. The thickness of the chondrocyte and extracellular matrix layer increased in the presence of the retinoid. Alkaline phosphatase activity and expression, proteoglycans synthesis, cbfa1 and type I collagen mRNA levels also increased in the presence of retinoic acid. These results demonstrated for the first time the proliferation, maturation of chondrocytes, and matrix deposition on MBCP, suggesting the potential for such scaffold in tissue engineering via the endochondral bone formation mechanism.

Original languageEnglish (US)
Pages (from-to)2283-2289
Number of pages7
JournalTissue Engineering
Volume12
Issue number8
DOIs
StatePublished - Aug 2006

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Growth Plate
Calcium phosphate
Chondrocytes
Scaffolds
Bone
Osteogenesis
Tissue engineering
Tissue Engineering
Tretinoin
Extracellular Matrix
Acids
Bioelectric potentials
Phosphatases
Cartilage
Scaffolds (biology)
Collagen
Bone Regeneration
Repair
Retinoids
Proteoglycans

ASJC Scopus subject areas

  • Biophysics
  • Biotechnology
  • Cell Biology

Cite this

Biphasic calcium phosphate : A scaffold for growth plate chondrocyte maturation. / Teixeira, Cristina; Nemelivsky, Yelena; Karkia, Claudia; Legeros, Racquel Z.

In: Tissue Engineering, Vol. 12, No. 8, 08.2006, p. 2283-2289.

Research output: Contribution to journalArticle

Teixeira, Cristina ; Nemelivsky, Yelena ; Karkia, Claudia ; Legeros, Racquel Z. / Biphasic calcium phosphate : A scaffold for growth plate chondrocyte maturation. In: Tissue Engineering. 2006 ; Vol. 12, No. 8. pp. 2283-2289.
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