Biomimetic emulsions reveal the effect of mechanical forces on cell-cell adhesion

Lea Laetitia Pontani, Ivane Jorjadze, Virgile Viasnoff, Jasna Brujic

    Research output: Contribution to journalArticle

    Abstract

    Cell-cell contacts in tissues are continuously subject to mechanical forces due to homeostatic pressure and active cytoskeleton dynamics. In the process of cellular adhesion, the molecular pathways are well characterized but the role of mechanics is less well understood. To isolate the role of pressure we present a dense packing of functionalized emulsion droplets in which surface interactions are tuned to mimic those of real cells. By visualizing the microstructure in 3D we find that a threshold compression force is necessary to overcome electrostatic repulsion and surface elasticity and establish protein-mediated adhesion. Varying the droplet interaction potential maps out a phase diagram for adhesion as a function of force and salt concentration. Remarkably, fitting the data with our theoretical model predicts binder concentrations in the adhesion areas that are similar to those found in real cells. Moreover, we quantify the dependence of the area of adhesion on the applied force and thus reveal adhesion strengthening with increasing external pressure even in the absence of active cellular processes. This biomimetic approach reveals a physical origin of pressure-sensitive adhesion and its strength across cell-cell junctions.

    Original languageEnglish (US)
    Pages (from-to)9839-9844
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume109
    Issue number25
    DOIs
    StatePublished - Jun 19 2012

    Fingerprint

    Biomimetics
    Emulsions
    Cell Adhesion
    Pressure
    Intercellular Junctions
    Elasticity
    Mechanics
    Cytoskeleton
    Static Electricity
    Theoretical Models
    Salts
    Proteins

    Keywords

    • Cell mechanics
    • Lipid emulsion
    • Protein emulsion

    ASJC Scopus subject areas

    • General

    Cite this

    Biomimetic emulsions reveal the effect of mechanical forces on cell-cell adhesion. / Pontani, Lea Laetitia; Jorjadze, Ivane; Viasnoff, Virgile; Brujic, Jasna.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 25, 19.06.2012, p. 9839-9844.

    Research output: Contribution to journalArticle

    Pontani, Lea Laetitia ; Jorjadze, Ivane ; Viasnoff, Virgile ; Brujic, Jasna. / Biomimetic emulsions reveal the effect of mechanical forces on cell-cell adhesion. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 25. pp. 9839-9844.
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