Binary regulation of Hippo pathway by Merlin/NF2, Kibra, Lgl, and melted specifies and maintains postmitotic neuronal fate

David Jukam, Claude Desplan

Research output: Contribution to journalArticle

Abstract

Patterning the Drosophila retina for color vision relies on postmitotic specification of photoreceptor subtypes. R8 photoreceptors express one of two light-sensing Rhodopsins, Rh5 or Rh6. This fate decision involves a bistable feedback loop between Melted, a PH-domain protein, and Warts, a kinase in the Hippo growth pathway. Here, we show that a subset of the Hippo pathway-Merlin, Kibra, and Lethal(2)giant larvae (Lgl), but not Expanded or Fat-is required for Warts expression and activity in R8 to specify Rh6 fate. Melted represses warts transcription to disrupt Hippo pathway activity and specify Rh5 fate. Therefore, R8 Hippo signaling exhibits ON-or-OFF regulation, promoting mutually exclusive fates. Furthermore, Merlin and Lgl are continuously required to maintain R8 neuronal subtypes. These results reveal roles for Merlin, Kibra, and Lgl in neuronal specification and maintenance and show that the Hippo pathway is reimplemented for sensory neuron fate by combining canonical and noncanonical regulatory steps.

Original languageEnglish (US)
Pages (from-to)874-887
Number of pages14
JournalDevelopmental Cell
Volume21
Issue number5
DOIs
StatePublished - Nov 15 2011

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Neurofibromin 2
Warts
Color vision
Specifications
Color Vision
Rhodopsin
Sensory Receptor Cells
Transcription
Drosophila
Neurons
Larva
Retina
Phosphotransferases
Fats
Maintenance
Feedback
Light
Growth
Proteins

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Binary regulation of Hippo pathway by Merlin/NF2, Kibra, Lgl, and melted specifies and maintains postmitotic neuronal fate. / Jukam, David; Desplan, Claude.

In: Developmental Cell, Vol. 21, No. 5, 15.11.2011, p. 874-887.

Research output: Contribution to journalArticle

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