Bilateral serotonergic projections to the dorsal hippocampus of the rat: simultaneous localization of 3H-5HT and HRP after retrograde transport

Research output: Contribution to journalArticle

Abstract

Horseradish peroxidase (HRP, Sigma VI, 30-70 nl of a 10-15% solution in saline) or 3H-5HT (30 Ci/mmole, 2.5 X 10 -3 M containing 3.3 X 10(-3) M norepinephrine in saline, 50-100 nl) was injected unilaterally into the dorsal hippocampus in separate groups of rats. HRP-labeled cells were seen in the hippocampus, medial septal nucleus, nucleus of the diagonal band, supramammilary nucleus, median raphe nucleus, interfascicular portion of the dorsal raphe nucleus, and the locus coeruleus. In contrast, 3H-5HT-labeled cells were largely restricted to the raphe nuclei. In this nucleus an equal number of ipsilateral and bilateral cells were found. Occasionally, these labeled cells stretched across the midline (bridge pattern). In another series, the 3H-5HT and HRP were injected into the same hippocampus either as a mixture or sequentially. This resulted in double labeling of the median and dorsal raphe neurons. A final group of rats received injections of 3H-5HT and HRP into opposing hippocampi. Double-labeled cells accounted for 10% of the neurons labeled. In addition, closely paired neurons composed of an HRP- and 3H-5HT-containing cell were found. In summary, the serotonergic fibers may play a key role in harmonizing the electrical activity of the hippocampi by use of bilateral projections, paired neurons with differential projections, and bridging neurons stretching across the midline but with unilateral projections.
Original languageUndefined
Pages (from-to)737-43
JournalJournal of Comparative Neurology
Volume203
Issue number4
DOIs
StatePublished - 1981

Keywords

  • Animals Axonal Transport Female Hippocampus/*metabolism Histocytochemistry Horseradish Peroxidase Male Neurons/metabolism Raphe Nuclei/metabolism Rats Rats, Inbred Strains Serotonin/*metabolism

Cite this

@article{f716901c4da944e2a3075ba9fb80c722,
title = "Bilateral serotonergic projections to the dorsal hippocampus of the rat: simultaneous localization of 3H-5HT and HRP after retrograde transport",
abstract = "Horseradish peroxidase (HRP, Sigma VI, 30-70 nl of a 10-15{\%} solution in saline) or 3H-5HT (30 Ci/mmole, 2.5 X 10 -3 M containing 3.3 X 10(-3) M norepinephrine in saline, 50-100 nl) was injected unilaterally into the dorsal hippocampus in separate groups of rats. HRP-labeled cells were seen in the hippocampus, medial septal nucleus, nucleus of the diagonal band, supramammilary nucleus, median raphe nucleus, interfascicular portion of the dorsal raphe nucleus, and the locus coeruleus. In contrast, 3H-5HT-labeled cells were largely restricted to the raphe nuclei. In this nucleus an equal number of ipsilateral and bilateral cells were found. Occasionally, these labeled cells stretched across the midline (bridge pattern). In another series, the 3H-5HT and HRP were injected into the same hippocampus either as a mixture or sequentially. This resulted in double labeling of the median and dorsal raphe neurons. A final group of rats received injections of 3H-5HT and HRP into opposing hippocampi. Double-labeled cells accounted for 10{\%} of the neurons labeled. In addition, closely paired neurons composed of an HRP- and 3H-5HT-containing cell were found. In summary, the serotonergic fibers may play a key role in harmonizing the electrical activity of the hippocampi by use of bilateral projections, paired neurons with differential projections, and bridging neurons stretching across the midline but with unilateral projections.",
keywords = "Animals Axonal Transport Female Hippocampus/*metabolism Histocytochemistry Horseradish Peroxidase Male Neurons/metabolism Raphe Nuclei/metabolism Rats Rats, Inbred Strains Serotonin/*metabolism",
author = "Azmitia, {E. C.}",
note = "Azmitia, E C Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. United states J Comp Neurol. 1981 Dec 20;203(4):737-43.",
year = "1981",
doi = "10.1002/cne.902030410",
language = "Undefined",
volume = "203",
pages = "737--43",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "4",

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TY - JOUR

T1 - Bilateral serotonergic projections to the dorsal hippocampus of the rat: simultaneous localization of 3H-5HT and HRP after retrograde transport

AU - Azmitia, E. C.

N1 - Azmitia, E C Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. United states J Comp Neurol. 1981 Dec 20;203(4):737-43.

PY - 1981

Y1 - 1981

N2 - Horseradish peroxidase (HRP, Sigma VI, 30-70 nl of a 10-15% solution in saline) or 3H-5HT (30 Ci/mmole, 2.5 X 10 -3 M containing 3.3 X 10(-3) M norepinephrine in saline, 50-100 nl) was injected unilaterally into the dorsal hippocampus in separate groups of rats. HRP-labeled cells were seen in the hippocampus, medial septal nucleus, nucleus of the diagonal band, supramammilary nucleus, median raphe nucleus, interfascicular portion of the dorsal raphe nucleus, and the locus coeruleus. In contrast, 3H-5HT-labeled cells were largely restricted to the raphe nuclei. In this nucleus an equal number of ipsilateral and bilateral cells were found. Occasionally, these labeled cells stretched across the midline (bridge pattern). In another series, the 3H-5HT and HRP were injected into the same hippocampus either as a mixture or sequentially. This resulted in double labeling of the median and dorsal raphe neurons. A final group of rats received injections of 3H-5HT and HRP into opposing hippocampi. Double-labeled cells accounted for 10% of the neurons labeled. In addition, closely paired neurons composed of an HRP- and 3H-5HT-containing cell were found. In summary, the serotonergic fibers may play a key role in harmonizing the electrical activity of the hippocampi by use of bilateral projections, paired neurons with differential projections, and bridging neurons stretching across the midline but with unilateral projections.

AB - Horseradish peroxidase (HRP, Sigma VI, 30-70 nl of a 10-15% solution in saline) or 3H-5HT (30 Ci/mmole, 2.5 X 10 -3 M containing 3.3 X 10(-3) M norepinephrine in saline, 50-100 nl) was injected unilaterally into the dorsal hippocampus in separate groups of rats. HRP-labeled cells were seen in the hippocampus, medial septal nucleus, nucleus of the diagonal band, supramammilary nucleus, median raphe nucleus, interfascicular portion of the dorsal raphe nucleus, and the locus coeruleus. In contrast, 3H-5HT-labeled cells were largely restricted to the raphe nuclei. In this nucleus an equal number of ipsilateral and bilateral cells were found. Occasionally, these labeled cells stretched across the midline (bridge pattern). In another series, the 3H-5HT and HRP were injected into the same hippocampus either as a mixture or sequentially. This resulted in double labeling of the median and dorsal raphe neurons. A final group of rats received injections of 3H-5HT and HRP into opposing hippocampi. Double-labeled cells accounted for 10% of the neurons labeled. In addition, closely paired neurons composed of an HRP- and 3H-5HT-containing cell were found. In summary, the serotonergic fibers may play a key role in harmonizing the electrical activity of the hippocampi by use of bilateral projections, paired neurons with differential projections, and bridging neurons stretching across the midline but with unilateral projections.

KW - Animals Axonal Transport Female Hippocampus/metabolism Histocytochemistry Horseradish Peroxidase Male Neurons/metabolism Raphe Nuclei/metabolism Rats Rats, Inbred Strains Serotonin/metabolism

U2 - 10.1002/cne.902030410

DO - 10.1002/cne.902030410

M3 - Article

VL - 203

SP - 737

EP - 743

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 4

ER -