Big data to the bench: Transcriptome analysis for undergraduates

Carl Procko, Steven Morrison, Courtney Dunar, Sara Mills, Brianna Maldonado, Carlee Cockrum, Nathan Emmanuel Peters, Shao Shan Carol Huang, Joanne Chory

Research output: Contribution to journalArticle

Abstract

Next-generation sequencing (NGS)-based methods are revolutionizing biology. Their prevalence requires biologists to be increasingly knowledgeable about computational methods to manage the enormous scale of data. As such, early introduction to NGS analysis and conceptual connection to wet-lab experiments is crucial for training young scientists. However, significant challenges impede the introduction of these methods into the undergraduate classroom, including the need for specialized computer programs and knowledge of computer coding. Here, we describe a semester-long, course-based undergraduate research experience at a liberal arts college combining RNA-sequencing (RNA-seq) analysis with student-driven, wet-lab experiments to investigate plant responses to light. Students derived hypotheses based on analysis of RNA-seq data and designed follow-up studies of gene expression and plant growth. Our assessments indicate that students acquired knowledge of big data analysis and computer coding; however, earlier exposure to computational methods may be beneficial. Our course requires minimal prior knowledge of plant biology, is easy to replicate, and can be modified to a shorter, directed-inquiry module. This framework promotes exploration of the links between gene expression and phenotype using examples that are clear and tractable and improves computational skills and bioinformatics self-efficacy to prepare students for the “big data” era of modern biology.

Original languageEnglish (US)
Article numberar19
JournalCBE life sciences education
Volume18
Issue number2
DOIs
StatePublished - Jun 1 2019

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ASJC Scopus subject areas

  • Education
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Procko, C., Morrison, S., Dunar, C., Mills, S., Maldonado, B., Cockrum, C., Peters, N. E., Huang, S. S. C., & Chory, J. (2019). Big data to the bench: Transcriptome analysis for undergraduates. CBE life sciences education, 18(2), [ar19]. https://doi.org/10.1187/cbe.18-08-0161