Bacillus subtilis spore resistance to simulated mars surface conditions

Marta Cortesão, Felix M. Fuchs, Fabian M. Commichau, Patrick Eichenberger, Andrew C. Schuerger, Wayne L. Nicholson, Peter Setlow, Ralf Moeller

Research output: Contribution to journalArticle

Abstract

In a Mars exploration scenario, knowing if and how highly resistant Bacillus subtilis spores would survive on the Martian surface is crucial to design planetary protection measures and avoid false positives in life-detection experiments. Therefore, in this study a systematic screening was performed to determine whether B. subtilis spores could survive an average day on Mars. For that, spores from two comprehensive sets of isogenic B. subtilis mutant strains, defective in DNA protection or repair genes, were exposed to 24 h of simulated Martian atmospheric environment with or without 8 h of Martian UV radiation [M(+)UV and M(-)UV, respectively]. When exposed to M(+)UV, spore survival was dependent on: (1) core dehydration maintenance, (2) protection of DNA by α/β-type small acid soluble proteins (SASP), and (3) removal and repair of the major UV photoproduct (SP) in spore DNA. In turn, when exposed to M(-)UV, spore survival was mainly dependent on protection by the multilayered spore coat, and DNA double-strand breaks represent the main lesion accumulated. Bacillus subtilis spores were able to survive for at least a limited time in a simulated Martian environment, both with or without solar UV radiation. Moreover, M(-)UV-treated spores exhibited survival rates significantly higher than the M(+)UV-treated spores. This suggests that on a real Martian surface, radiation shielding of spores (e.g., by dust, rocks, or spacecraft surface irregularities) might significantly extend survival rates. Mutagenesis were strongly dependent on the functionality of all structural components with small acid-soluble spore proteins, coat layers and dipicolinic acid as key protectants and efficiency DNA damage removal by AP endonucleases (ExoA and Nfo), non-homologous end joining (NHEJ), mismatch repair (MMR) and error-prone translesion synthesis (TLS). Thus, future efforts should focus on: (1) determining the DNA damage in wild-type spores exposed to M(+/-)UV and (2) assessing spore survival and viability with shielding of spores via Mars regolith and other relevant materials.

Original languageEnglish (US)
Article number333
JournalFrontiers in Microbiology
Volume10
Issue numberFEB
DOIs
StatePublished - Jan 1 2019

Fingerprint

Mars
Bacillus subtilis
Spores
Radiation
DNA Damage
DNA
Spacecraft
DNA-(Apurinic or Apyrimidinic Site) Lyase
Acids
DNA Mismatch Repair
Double-Stranded DNA Breaks
Capsid Proteins
Dust
Dehydration

Keywords

  • Bacillus subtilis
  • Contamination
  • DNA repair
  • Mars
  • Planetary protection
  • Radiation
  • SASP
  • Spore resistance

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Cortesão, M., Fuchs, F. M., Commichau, F. M., Eichenberger, P., Schuerger, A. C., Nicholson, W. L., ... Moeller, R. (2019). Bacillus subtilis spore resistance to simulated mars surface conditions. Frontiers in Microbiology, 10(FEB), [333]. https://doi.org/10.3389/fmicb.2019.00333

Bacillus subtilis spore resistance to simulated mars surface conditions. / Cortesão, Marta; Fuchs, Felix M.; Commichau, Fabian M.; Eichenberger, Patrick; Schuerger, Andrew C.; Nicholson, Wayne L.; Setlow, Peter; Moeller, Ralf.

In: Frontiers in Microbiology, Vol. 10, No. FEB, 333, 01.01.2019.

Research output: Contribution to journalArticle

Cortesão, M, Fuchs, FM, Commichau, FM, Eichenberger, P, Schuerger, AC, Nicholson, WL, Setlow, P & Moeller, R 2019, 'Bacillus subtilis spore resistance to simulated mars surface conditions', Frontiers in Microbiology, vol. 10, no. FEB, 333. https://doi.org/10.3389/fmicb.2019.00333
Cortesão, Marta ; Fuchs, Felix M. ; Commichau, Fabian M. ; Eichenberger, Patrick ; Schuerger, Andrew C. ; Nicholson, Wayne L. ; Setlow, Peter ; Moeller, Ralf. / Bacillus subtilis spore resistance to simulated mars surface conditions. In: Frontiers in Microbiology. 2019 ; Vol. 10, No. FEB.
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