Autoregulation of fetal serotonergic neuronal development: Role of high affinity serotonin receptors

Patricia M. Whitaker-Azmitia, Efrain C. Azmitia

Research output: Contribution to journalArticle

Abstract

A microculture technique was used to study the factors regulating the development of fetal rat serotonergic neurons. Mesencephalic raphe cells from E14 rats co-cultured with hippocampal cells from E18 were grown for up to 4 days in the presence of various agents known to alter serotonergic function in the mature brain. Pargyline (a non-specific monoamine oxidase inhibitor) alone and with serotonin (10-8 to 10-6 M) both inhibited growth of serotonergic neurons as assessed by uptake of [3H]serotonin. 5-methoxytryptamine (5-MT), a serotonin agonist with selectivity for serotonin autoreceptors, inhibited growth at low concentrations, but this inhibition was overcome at higher concentrations. Using immunocytochemistry with a primary anti-serotonin antibody, 5-MT was observed to produce stunted processes, increase autoinnervation and lead to neuronal death. A model is proposed whereby high affinity serotonin receptors in fetal brainstem tissue and in fetal forebrain tissue regulate direction and extent of growth. We have confirmed the presence of these receptors using a direct binding assay.

Original languageEnglish (US)
Pages (from-to)307-312
Number of pages6
JournalNeuroscience Letters
Volume67
Issue number3
DOIs
StatePublished - Jun 30 1986

Fingerprint

Serotonin Receptors
Serotonin
Homeostasis
5-Methoxytryptamine
Serotonergic Neurons
Fetus
Growth
Pargyline
Autoreceptors
Serotonin Receptor Agonists
Monoamine Oxidase Inhibitors
Prosencephalon
Fetal Development
Brain Stem
Anti-Idiotypic Antibodies
Immunohistochemistry
Brain

Keywords

  • autoreceptor
  • neurodevelopment
  • serotonin
  • serotonin receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Autoregulation of fetal serotonergic neuronal development : Role of high affinity serotonin receptors. / Whitaker-Azmitia, Patricia M.; Azmitia, Efrain C.

In: Neuroscience Letters, Vol. 67, No. 3, 30.06.1986, p. 307-312.

Research output: Contribution to journalArticle

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