Atomic structure of a tryptophan-zipper pentamer

Jie Liu, Wei Yong, Yiqun Deng, Neville R. Kallenbach, Min Lu

Research output: Contribution to journalArticle

Abstract

Coiled-coil motifs are ubiquitous mediators of specific protein-protein interactions through the formation of interlocking hydrophobic seams between α-helical chains. Residues that form these seams occur at the first (a) and fourth (d) positions of a characteristic 7-aa repeat and are primarily aliphatic. The potential of aromatic residues to promote helix association in a coiled coil was explored by engineering a "Trp-zipper" protein with Trp residues at all 14 a and d positions. The protein forms a discrete, stable, α-helical pentamer in water at physiological pH. Its 1.45-Å crystal structure reveals a parallel, five-stranded coiled coil, a previously uncharacterized type of "knobs-into-holes" packing interaction between interfacial Trp side chains, and an unusual ≈8-Å-diameter axial channel lined with indole rings that is filled with polyethylene glycol 400 and water and sulfate ion molecules. The engineered Trp-zipper pentamer enlarges current views of coiled-coil assembly, molecular recognition, and protein engineering, and may serve as a soluble model for membrane ion channels.

Original languageEnglish (US)
Pages (from-to)16156-16161
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number46
DOIs
StatePublished - Nov 16 2004

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Tryptophan
Ion Channels
Proteins
Protein Engineering
Water
Sulfates
Ions

Keywords

  • Coiled coils
  • Protein design
  • Protein structure

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Atomic structure of a tryptophan-zipper pentamer. / Liu, Jie; Yong, Wei; Deng, Yiqun; Kallenbach, Neville R.; Lu, Min.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 46, 16.11.2004, p. 16156-16161.

Research output: Contribution to journalArticle

Liu, Jie ; Yong, Wei ; Deng, Yiqun ; Kallenbach, Neville R. ; Lu, Min. / Atomic structure of a tryptophan-zipper pentamer. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 46. pp. 16156-16161.
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