Association between trichomonas vaginalis and vaginal bacterial community composition among reproductive-age women

Rebecca M. Brotman, L. Latey Bradford, Melissa Conrad, Pawel Gajer, Kevin Ault, Ligia Peralta, Larry J. Forney, Jane M. Carlton, Zaid Abdo, Jacques Ravel

Research output: Contribution to journalArticle

Abstract

OBJECTIVES: Some vaginal bacterial communities are thought to prevent infection by sexually transmitted organisms. Prior work demonstrated that the vaginal microbiota of reproductive-age women cluster into 5 types of bacterial communities; 4 dominated by Lactobacillus species (L. iners, L. crispatus, L. gasseri, L. jensenii) and 1 (termed community state type (CST) IV) lacking significant numbers of lactobacilli and characterized by higher proportions of Atopobium, Prevotella, Parvimonas, Sneathia, Gardnerella, Mobiluncus, and other taxa. We sought to evaluate the relationship between vaginal bacterial composition and Trichomonas vaginalis. METHODS: Self-collected vaginal swabs were obtained cross-sectionally from 394 women equally representing 4 ethnic/racial groups. T. vaginalis screening was performed using PCR targeting the 18S rRNA and β-tubulin genes. Vaginal bacterial composition was characterized by pyrosequencing of barcoded 16S rRNA genes. A panel of 11 microsatellite markers was used to genotype T. vaginalis. The association between vaginal microbiota and T. vaginalis was evaluated by exact logistic regression. RESULTS: T. vaginalis was detected in 2.8% of participants (11/394). Of the 11 T. vaginalis-positive cases, 8 (72%) were categorized as CST-IV, 2 (18%) as communities dominated by L. iners, and 1 (9%) as L. crispatus-dominated (P = 0.05). CST-IV microbiota were associated with an 8-fold increased odds of detecting T. vaginalis compared with women in the L. crispatus-dominated state (OR: 8.26, 95% CI: 1.07-372.65). Seven of the 11 T. vaginalis isolates were assigned to 2 genotypes. CONCLUSION: T. vaginalis was associated with vaginal microbiota consisting of low proportions of lactobacilli and high proportions of Mycoplasma, Parvimonas, Sneathia, and other anaerobes.

Original languageEnglish (US)
Pages (from-to)807-812
Number of pages6
JournalSexually Transmitted Diseases
Volume39
Issue number10
DOIs
StatePublished - Oct 2012

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Trichomonas vaginalis
Microbiota
Lactobacillus
rRNA Genes
Gardnerella
Mobiluncus
Genotype
Prevotella
Mycoplasma
Tubulin
Sexually Transmitted Diseases
Ethnic Groups
Microsatellite Repeats
Logistic Models
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Dermatology
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)
  • Infectious Diseases

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Association between trichomonas vaginalis and vaginal bacterial community composition among reproductive-age women. / Brotman, Rebecca M.; Bradford, L. Latey; Conrad, Melissa; Gajer, Pawel; Ault, Kevin; Peralta, Ligia; Forney, Larry J.; Carlton, Jane M.; Abdo, Zaid; Ravel, Jacques.

In: Sexually Transmitted Diseases, Vol. 39, No. 10, 10.2012, p. 807-812.

Research output: Contribution to journalArticle

Brotman, RM, Bradford, LL, Conrad, M, Gajer, P, Ault, K, Peralta, L, Forney, LJ, Carlton, JM, Abdo, Z & Ravel, J 2012, 'Association between trichomonas vaginalis and vaginal bacterial community composition among reproductive-age women', Sexually Transmitted Diseases, vol. 39, no. 10, pp. 807-812. https://doi.org/10.1097/OLQ.0b013e3182631c79
Brotman, Rebecca M. ; Bradford, L. Latey ; Conrad, Melissa ; Gajer, Pawel ; Ault, Kevin ; Peralta, Ligia ; Forney, Larry J. ; Carlton, Jane M. ; Abdo, Zaid ; Ravel, Jacques. / Association between trichomonas vaginalis and vaginal bacterial community composition among reproductive-age women. In: Sexually Transmitted Diseases. 2012 ; Vol. 39, No. 10. pp. 807-812.
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title = "Association between trichomonas vaginalis and vaginal bacterial community composition among reproductive-age women",
abstract = "OBJECTIVES: Some vaginal bacterial communities are thought to prevent infection by sexually transmitted organisms. Prior work demonstrated that the vaginal microbiota of reproductive-age women cluster into 5 types of bacterial communities; 4 dominated by Lactobacillus species (L. iners, L. crispatus, L. gasseri, L. jensenii) and 1 (termed community state type (CST) IV) lacking significant numbers of lactobacilli and characterized by higher proportions of Atopobium, Prevotella, Parvimonas, Sneathia, Gardnerella, Mobiluncus, and other taxa. We sought to evaluate the relationship between vaginal bacterial composition and Trichomonas vaginalis. METHODS: Self-collected vaginal swabs were obtained cross-sectionally from 394 women equally representing 4 ethnic/racial groups. T. vaginalis screening was performed using PCR targeting the 18S rRNA and β-tubulin genes. Vaginal bacterial composition was characterized by pyrosequencing of barcoded 16S rRNA genes. A panel of 11 microsatellite markers was used to genotype T. vaginalis. The association between vaginal microbiota and T. vaginalis was evaluated by exact logistic regression. RESULTS: T. vaginalis was detected in 2.8{\%} of participants (11/394). Of the 11 T. vaginalis-positive cases, 8 (72{\%}) were categorized as CST-IV, 2 (18{\%}) as communities dominated by L. iners, and 1 (9{\%}) as L. crispatus-dominated (P = 0.05). CST-IV microbiota were associated with an 8-fold increased odds of detecting T. vaginalis compared with women in the L. crispatus-dominated state (OR: 8.26, 95{\%} CI: 1.07-372.65). Seven of the 11 T. vaginalis isolates were assigned to 2 genotypes. CONCLUSION: T. vaginalis was associated with vaginal microbiota consisting of low proportions of lactobacilli and high proportions of Mycoplasma, Parvimonas, Sneathia, and other anaerobes.",
author = "Brotman, {Rebecca M.} and Bradford, {L. Latey} and Melissa Conrad and Pawel Gajer and Kevin Ault and Ligia Peralta and Forney, {Larry J.} and Carlton, {Jane M.} and Zaid Abdo and Jacques Ravel",
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AU - Brotman, Rebecca M.

AU - Bradford, L. Latey

AU - Conrad, Melissa

AU - Gajer, Pawel

AU - Ault, Kevin

AU - Peralta, Ligia

AU - Forney, Larry J.

AU - Carlton, Jane M.

AU - Abdo, Zaid

AU - Ravel, Jacques

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N2 - OBJECTIVES: Some vaginal bacterial communities are thought to prevent infection by sexually transmitted organisms. Prior work demonstrated that the vaginal microbiota of reproductive-age women cluster into 5 types of bacterial communities; 4 dominated by Lactobacillus species (L. iners, L. crispatus, L. gasseri, L. jensenii) and 1 (termed community state type (CST) IV) lacking significant numbers of lactobacilli and characterized by higher proportions of Atopobium, Prevotella, Parvimonas, Sneathia, Gardnerella, Mobiluncus, and other taxa. We sought to evaluate the relationship between vaginal bacterial composition and Trichomonas vaginalis. METHODS: Self-collected vaginal swabs were obtained cross-sectionally from 394 women equally representing 4 ethnic/racial groups. T. vaginalis screening was performed using PCR targeting the 18S rRNA and β-tubulin genes. Vaginal bacterial composition was characterized by pyrosequencing of barcoded 16S rRNA genes. A panel of 11 microsatellite markers was used to genotype T. vaginalis. The association between vaginal microbiota and T. vaginalis was evaluated by exact logistic regression. RESULTS: T. vaginalis was detected in 2.8% of participants (11/394). Of the 11 T. vaginalis-positive cases, 8 (72%) were categorized as CST-IV, 2 (18%) as communities dominated by L. iners, and 1 (9%) as L. crispatus-dominated (P = 0.05). CST-IV microbiota were associated with an 8-fold increased odds of detecting T. vaginalis compared with women in the L. crispatus-dominated state (OR: 8.26, 95% CI: 1.07-372.65). Seven of the 11 T. vaginalis isolates were assigned to 2 genotypes. CONCLUSION: T. vaginalis was associated with vaginal microbiota consisting of low proportions of lactobacilli and high proportions of Mycoplasma, Parvimonas, Sneathia, and other anaerobes.

AB - OBJECTIVES: Some vaginal bacterial communities are thought to prevent infection by sexually transmitted organisms. Prior work demonstrated that the vaginal microbiota of reproductive-age women cluster into 5 types of bacterial communities; 4 dominated by Lactobacillus species (L. iners, L. crispatus, L. gasseri, L. jensenii) and 1 (termed community state type (CST) IV) lacking significant numbers of lactobacilli and characterized by higher proportions of Atopobium, Prevotella, Parvimonas, Sneathia, Gardnerella, Mobiluncus, and other taxa. We sought to evaluate the relationship between vaginal bacterial composition and Trichomonas vaginalis. METHODS: Self-collected vaginal swabs were obtained cross-sectionally from 394 women equally representing 4 ethnic/racial groups. T. vaginalis screening was performed using PCR targeting the 18S rRNA and β-tubulin genes. Vaginal bacterial composition was characterized by pyrosequencing of barcoded 16S rRNA genes. A panel of 11 microsatellite markers was used to genotype T. vaginalis. The association between vaginal microbiota and T. vaginalis was evaluated by exact logistic regression. RESULTS: T. vaginalis was detected in 2.8% of participants (11/394). Of the 11 T. vaginalis-positive cases, 8 (72%) were categorized as CST-IV, 2 (18%) as communities dominated by L. iners, and 1 (9%) as L. crispatus-dominated (P = 0.05). CST-IV microbiota were associated with an 8-fold increased odds of detecting T. vaginalis compared with women in the L. crispatus-dominated state (OR: 8.26, 95% CI: 1.07-372.65). Seven of the 11 T. vaginalis isolates were assigned to 2 genotypes. CONCLUSION: T. vaginalis was associated with vaginal microbiota consisting of low proportions of lactobacilli and high proportions of Mycoplasma, Parvimonas, Sneathia, and other anaerobes.

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