Abstract
In this study, it is shown that the chemical exchange saturation transfer (CEST) method for hydroxyl protons can be used to detect changes in glycosaminoglycan (GAG) concentration in the intervertebral disc. The method, termed gagCEST, was demonstrated ex vivo by correlating the CEST effect with the fixed charge density (FCD) of the nucleus pulposus (NP), as well as by correlating tissue CEST images with their corresponding 23Na images. Incubation of five NP samples with trypsin produced samples with varying GAG content (n=19). A good correlation was found between the -OH CEST effect and FCD, as well as with the N-acetyl signal amplitude. gagCEST imagesinvitro further illustrated the amount of detail obtainable from this contrast mechanism when compared with conventional imaging. The large concentration of GAG and the relatively long T 1 of water in NP make the method sensitive, in particular, for the assessment of GAG depletion in this tissue. It is the loss of GAG in NP that indicates the early stage of disc degeneration.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 255-261 |
| Number of pages | 7 |
| Journal | NMR in Biomedicine |
| Volume | 25 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2012 |
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Keywords
- Chemical exchange saturation transfer
- Glycosaminoglycan
- Intervertebral disc
- MRI
- Nucleus pulposus
- Proteoglycans
ASJC Scopus subject areas
- Spectroscopy
- Molecular Medicine
- Radiology Nuclear Medicine and imaging
Cite this
Assessment of glycosaminoglycan concentration changes in the intervertebral disc via chemical exchange saturation transfer. / Saar, Galit; Zhang, Boyang; Ling, Wen; Regatte, Ravinder R.; Navon, Gil; Jerschow, Alexej.
In: NMR in Biomedicine, Vol. 25, No. 2, 02.2012, p. 255-261.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Assessment of glycosaminoglycan concentration changes in the intervertebral disc via chemical exchange saturation transfer
AU - Saar, Galit
AU - Zhang, Boyang
AU - Ling, Wen
AU - Regatte, Ravinder R.
AU - Navon, Gil
AU - Jerschow, Alexej
PY - 2012/2
Y1 - 2012/2
N2 - In this study, it is shown that the chemical exchange saturation transfer (CEST) method for hydroxyl protons can be used to detect changes in glycosaminoglycan (GAG) concentration in the intervertebral disc. The method, termed gagCEST, was demonstrated ex vivo by correlating the CEST effect with the fixed charge density (FCD) of the nucleus pulposus (NP), as well as by correlating tissue CEST images with their corresponding 23Na images. Incubation of five NP samples with trypsin produced samples with varying GAG content (n=19). A good correlation was found between the -OH CEST effect and FCD, as well as with the N-acetyl signal amplitude. gagCEST imagesinvitro further illustrated the amount of detail obtainable from this contrast mechanism when compared with conventional imaging. The large concentration of GAG and the relatively long T 1 of water in NP make the method sensitive, in particular, for the assessment of GAG depletion in this tissue. It is the loss of GAG in NP that indicates the early stage of disc degeneration.
AB - In this study, it is shown that the chemical exchange saturation transfer (CEST) method for hydroxyl protons can be used to detect changes in glycosaminoglycan (GAG) concentration in the intervertebral disc. The method, termed gagCEST, was demonstrated ex vivo by correlating the CEST effect with the fixed charge density (FCD) of the nucleus pulposus (NP), as well as by correlating tissue CEST images with their corresponding 23Na images. Incubation of five NP samples with trypsin produced samples with varying GAG content (n=19). A good correlation was found between the -OH CEST effect and FCD, as well as with the N-acetyl signal amplitude. gagCEST imagesinvitro further illustrated the amount of detail obtainable from this contrast mechanism when compared with conventional imaging. The large concentration of GAG and the relatively long T 1 of water in NP make the method sensitive, in particular, for the assessment of GAG depletion in this tissue. It is the loss of GAG in NP that indicates the early stage of disc degeneration.
KW - Chemical exchange saturation transfer
KW - Glycosaminoglycan
KW - Intervertebral disc
KW - MRI
KW - Nucleus pulposus
KW - Proteoglycans
UR - http://www.scopus.com/inward/record.url?scp=84856010278&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856010278&partnerID=8YFLogxK
U2 - 10.1002/nbm.1741
DO - 10.1002/nbm.1741
M3 - Article
C2 - 22253087
AN - SCOPUS:84856010278
VL - 25
SP - 255
EP - 261
JO - NMR in Biomedicine
JF - NMR in Biomedicine
SN - 0952-3480
IS - 2
ER -