Artificial protein block polymer libraries bearing two SADs

Effects of elastin domain repeats

Min Dai, Jennifer Haghpanah, Navjot Singh, Eric W. Roth, Alice Liang, Raymond S. Tu, Jin Montclare

Research output: Contribution to journalArticle

Abstract

We have generated protein block polymer EnC and CE n libraries composed of two different self-assembling domains (SADs) derived from elastin (E) and the cartilage oligomeric matrix protein coiled-coil (C). As the E domain is shortened, the polymers exhibit an increase in inverse transition temperature (Tt); however, the range of temperature change differs dramatically between the EnC and CE n library. Whereas all polymers assemble into nanoparticles, the bulk mechanical properties of the EnC are very different from CEn. The EnC members demonstrate viscolelastic behavior under ambient conditions and assemble into elastic soft gels above their Tt values. By contrast, the CE n members are predominantly viscous at all temperatures. All library members demonstrate binding to curcumin. The differential thermoresponsive behaviors of the EnC and CE n libraries in addition to their small molecule recognition abilities make them suitable for potential use in tissue engineering and drug delivery.

Original languageEnglish (US)
Pages (from-to)4240-4246
Number of pages7
JournalBiomacromolecules
Volume12
Issue number12
DOIs
StatePublished - Dec 12 2011

Fingerprint

Bearings (structural)
Elastin
Block copolymers
Proteins
Superconducting transition temperature
Polymers
Cartilage Oligomeric Matrix Protein
Curcumin
Bioelectric potentials
Cartilage
Drug delivery
Tissue engineering
Gels
Nanoparticles
Mechanical properties
Temperature
Molecules

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials

Cite this

Artificial protein block polymer libraries bearing two SADs : Effects of elastin domain repeats. / Dai, Min; Haghpanah, Jennifer; Singh, Navjot; Roth, Eric W.; Liang, Alice; Tu, Raymond S.; Montclare, Jin.

In: Biomacromolecules, Vol. 12, No. 12, 12.12.2011, p. 4240-4246.

Research output: Contribution to journalArticle

Dai, Min ; Haghpanah, Jennifer ; Singh, Navjot ; Roth, Eric W. ; Liang, Alice ; Tu, Raymond S. ; Montclare, Jin. / Artificial protein block polymer libraries bearing two SADs : Effects of elastin domain repeats. In: Biomacromolecules. 2011 ; Vol. 12, No. 12. pp. 4240-4246.
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