Aromatic DNA adducts in foundry workers in relation to exposure, life style and CYP1A1 and glutathione transferase M1 genotype

Kari Hemminki, Chris Dickey, Svante Karlsson, Doug Bell, Yanzhi Hsu, Wei Yann Tsai, LaVerne A. Mooney, Kirsti Savela, Frederica P. Perera

Research output: Contribution to journalArticle

Abstract

Levels of aromatic DNA adducts in foundry workers and controls were followed at four annual samplings. During this time exposure to polycyclic aromatic hydrocarbons (PAH) decreased and the level of DNA adducts decreased accordingly. In the total group exposure was related to the level of adducts. Adduct levels correlated with urinary 1-hydroxypyrene (LOGU1OH), air benzo[a]pyrene, weekly working hours and daily cigarette consumption. In a multivariate model 1-hydroxypyrene had a consistent effect. Neither glutathione transferase M1 (GSTM1) nor cytochrome P450 1A1 (CYP1A1) genotypes had clear effects. Yet the individuals lacking GSTM1 had a stronger effect of LOGU1OH and some effect by other sources of PAH, such as charcoal broiled food, although all these variables were not significant in the multivariate model. The rare individuals with a CYP1A1 polymorphism MspI containing an amino acid change at isoleucine had an increased level of adducts. The results showed that the postlabelling method used was able to detect an increase in aromatic DNA adducts in leukocytes when exposure to benzo[a]pyrene in air was ~5 ng/m3. At such low levels smoking and charcoal broiled food may be important contributors to adducts.

Original languageEnglish (US)
Pages (from-to)345-350
Number of pages6
JournalCarcinogenesis
Volume18
Issue number2
DOIs
StatePublished - Feb 1997

Fingerprint

DNA Adducts
Glutathione Transferase
Cytochrome P-450 Enzyme System
Life Style
Charcoal
Benzo(a)pyrene
Polycyclic Aromatic Hydrocarbons
Genotype
Air
Food
Isoleucine
Tobacco Products
Leukocytes
Smoking
Amino Acids
1-hydroxypyrene

ASJC Scopus subject areas

  • Cancer Research

Cite this

Aromatic DNA adducts in foundry workers in relation to exposure, life style and CYP1A1 and glutathione transferase M1 genotype. / Hemminki, Kari; Dickey, Chris; Karlsson, Svante; Bell, Doug; Hsu, Yanzhi; Tsai, Wei Yann; Mooney, LaVerne A.; Savela, Kirsti; Perera, Frederica P.

In: Carcinogenesis, Vol. 18, No. 2, 02.1997, p. 345-350.

Research output: Contribution to journalArticle

Hemminki, K, Dickey, C, Karlsson, S, Bell, D, Hsu, Y, Tsai, WY, Mooney, LA, Savela, K & Perera, FP 1997, 'Aromatic DNA adducts in foundry workers in relation to exposure, life style and CYP1A1 and glutathione transferase M1 genotype', Carcinogenesis, vol. 18, no. 2, pp. 345-350. https://doi.org/10.1093/carcin/18.2.345
Hemminki, Kari ; Dickey, Chris ; Karlsson, Svante ; Bell, Doug ; Hsu, Yanzhi ; Tsai, Wei Yann ; Mooney, LaVerne A. ; Savela, Kirsti ; Perera, Frederica P. / Aromatic DNA adducts in foundry workers in relation to exposure, life style and CYP1A1 and glutathione transferase M1 genotype. In: Carcinogenesis. 1997 ; Vol. 18, No. 2. pp. 345-350.
@article{4d793712e2724a8ab33dcafccaab6936,
title = "Aromatic DNA adducts in foundry workers in relation to exposure, life style and CYP1A1 and glutathione transferase M1 genotype",
abstract = "Levels of aromatic DNA adducts in foundry workers and controls were followed at four annual samplings. During this time exposure to polycyclic aromatic hydrocarbons (PAH) decreased and the level of DNA adducts decreased accordingly. In the total group exposure was related to the level of adducts. Adduct levels correlated with urinary 1-hydroxypyrene (LOGU1OH), air benzo[a]pyrene, weekly working hours and daily cigarette consumption. In a multivariate model 1-hydroxypyrene had a consistent effect. Neither glutathione transferase M1 (GSTM1) nor cytochrome P450 1A1 (CYP1A1) genotypes had clear effects. Yet the individuals lacking GSTM1 had a stronger effect of LOGU1OH and some effect by other sources of PAH, such as charcoal broiled food, although all these variables were not significant in the multivariate model. The rare individuals with a CYP1A1 polymorphism MspI containing an amino acid change at isoleucine had an increased level of adducts. The results showed that the postlabelling method used was able to detect an increase in aromatic DNA adducts in leukocytes when exposure to benzo[a]pyrene in air was ~5 ng/m3. At such low levels smoking and charcoal broiled food may be important contributors to adducts.",
author = "Kari Hemminki and Chris Dickey and Svante Karlsson and Doug Bell and Yanzhi Hsu and Tsai, {Wei Yann} and Mooney, {LaVerne A.} and Kirsti Savela and Perera, {Frederica P.}",
year = "1997",
month = "2",
doi = "10.1093/carcin/18.2.345",
language = "English (US)",
volume = "18",
pages = "345--350",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Aromatic DNA adducts in foundry workers in relation to exposure, life style and CYP1A1 and glutathione transferase M1 genotype

AU - Hemminki, Kari

AU - Dickey, Chris

AU - Karlsson, Svante

AU - Bell, Doug

AU - Hsu, Yanzhi

AU - Tsai, Wei Yann

AU - Mooney, LaVerne A.

AU - Savela, Kirsti

AU - Perera, Frederica P.

PY - 1997/2

Y1 - 1997/2

N2 - Levels of aromatic DNA adducts in foundry workers and controls were followed at four annual samplings. During this time exposure to polycyclic aromatic hydrocarbons (PAH) decreased and the level of DNA adducts decreased accordingly. In the total group exposure was related to the level of adducts. Adduct levels correlated with urinary 1-hydroxypyrene (LOGU1OH), air benzo[a]pyrene, weekly working hours and daily cigarette consumption. In a multivariate model 1-hydroxypyrene had a consistent effect. Neither glutathione transferase M1 (GSTM1) nor cytochrome P450 1A1 (CYP1A1) genotypes had clear effects. Yet the individuals lacking GSTM1 had a stronger effect of LOGU1OH and some effect by other sources of PAH, such as charcoal broiled food, although all these variables were not significant in the multivariate model. The rare individuals with a CYP1A1 polymorphism MspI containing an amino acid change at isoleucine had an increased level of adducts. The results showed that the postlabelling method used was able to detect an increase in aromatic DNA adducts in leukocytes when exposure to benzo[a]pyrene in air was ~5 ng/m3. At such low levels smoking and charcoal broiled food may be important contributors to adducts.

AB - Levels of aromatic DNA adducts in foundry workers and controls were followed at four annual samplings. During this time exposure to polycyclic aromatic hydrocarbons (PAH) decreased and the level of DNA adducts decreased accordingly. In the total group exposure was related to the level of adducts. Adduct levels correlated with urinary 1-hydroxypyrene (LOGU1OH), air benzo[a]pyrene, weekly working hours and daily cigarette consumption. In a multivariate model 1-hydroxypyrene had a consistent effect. Neither glutathione transferase M1 (GSTM1) nor cytochrome P450 1A1 (CYP1A1) genotypes had clear effects. Yet the individuals lacking GSTM1 had a stronger effect of LOGU1OH and some effect by other sources of PAH, such as charcoal broiled food, although all these variables were not significant in the multivariate model. The rare individuals with a CYP1A1 polymorphism MspI containing an amino acid change at isoleucine had an increased level of adducts. The results showed that the postlabelling method used was able to detect an increase in aromatic DNA adducts in leukocytes when exposure to benzo[a]pyrene in air was ~5 ng/m3. At such low levels smoking and charcoal broiled food may be important contributors to adducts.

UR - http://www.scopus.com/inward/record.url?scp=0031408924&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031408924&partnerID=8YFLogxK

U2 - 10.1093/carcin/18.2.345

DO - 10.1093/carcin/18.2.345

M3 - Article

VL - 18

SP - 345

EP - 350

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 2

ER -